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Prepublished online as a Blood First Edition Paper on January 2, 2003; DOI 10.1182/blood-2002-11-3456. This Article Full Text (PDF) All Versions of this Article: 2002-11-3456v1 101/10/4042    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Camacho, F. I Articles by Piris, M. A Search for Related Content PubMed PubMed Citation Articles by Camacho, F. I Articles by Piris, M. A Social Bookmarking                   What's this? Submitted November 15, 2002 Accepted December 23, 2002 Molecular heterogeneity in MCL, defined by the use of specific VH genes and the frequency of somatic mutations Francisca I Camacho, Patrocinio Algara, Antonia Rodriguez, Elena Ruiz-Ballesteros, Manuela Mollejo, Nerea Martinez, Jose A Martinez-Climent, Marcos Gonzalez, Marisol Mateo, Alessia Caleo, Margarita Sanchez-Beato, Javier Menarguez, Javier Garcia-Conde, Francesc Sole, Elias Campo, and Miguel A Piris* Molecular Pathology Program, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain Department of Genetics and Pathology, Hospital Virgen de la Salud, Toledo, Spain Department of Haematology, Hospital Clinico, Valencia, Spain Department of Haematology, Hospital Clinico, Salamanca, Spain Laboratory of Citologia Hematologica, Hospital del Mar, Barcelona, Spain Department of Pathology, Hospital Clinic, Barcelona, Spain Department of Pathology, Hospital Gregorio Maranon, Madrid, Spain * Corresponding author; email: mapiris{at}cnio.es. This study aimed to explores whether the presence of somatic mutations, or a biased use of IgVH genes were associated related with the clinical features in a series of 96 cases of Mantle Cell Lymphoma (MCL). The cases were studied by semi-nested PCR using primers from the FR1 and JH regions. There was results showed an unexpectedly high frequency of somatic mutations, with 29/103 sequences showing more than 2% of mutations. Additionally a bBiased usage of specific VH segments was also found:identified. Thus, the most more widely used genes used in this series were VH3-21 (10 cases), VH3-23 (9), VH4-34 (11) and VH4-59 (9). VH mutational frequency, taking into account different thresholds, did not discriminatetinguishd among different OS overall survival probabilities. Nevertheless, a more frequent use of VH3-21 or VH4-59 (8/18) was observed in the group of long-term survivors (18 cases > 5 yearsrs) (p<0.01). None of these long-term survivors cases presented the VH3-23 gene rearrangement. As in other lymphoproliferative disorders, the expression of CD38 and/or p53 was associated with a worse poorer survival probability. This unon-random usage of IgVH segments suggests that specific antigens may could play a pathogenically relevant role in the genesis or progression of subsets of MCL cases, and may help in distinguishing a significant group of MCL long-term survivors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Gao, L. Peterson, B. Nelson, C. Goolsby, and Y.-H. Chen Immunophenotypic Variations in Mantle Cell Lymphoma Am J Clin Pathol, November 1, 2009; 132(5): 699 - 706. [Abstract] [Full Text] [PDF] L. Tracey, M. Aggarwal, M. Garcia-Cosio, R. Villuendas, P. Algara, M. Sanchez-Beato, A. Sanchez-Aguilera, J. F. Garcia, A. Rodriguez, F. I. Camacho, et al. 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