|
|
Prepublished online as a Blood First Edition Paper on February 27, 2003; DOI 10.1182/blood-2002-11-3483.
Submitted November 19, 2002
Accepted February 14, 2003
Involvement of a CD47-dependent pathway in platelet adhesion on inflamed vascular endothelium under flow
Patricia Lagadec, Olivier Dejoux, Michel Ticchioni, Francoise Cottrez, Mette Johansen, Eric J Brown, and Alain Bernard*
Laboratory of Immunology, INSERM, Unite 343, Nice, France
Center for Host/Pathogen Interactions, University of California, San Francisco, CA, USA
* Corresponding author; email: abernard{at}unice.fr.
Resting platelet adhesion to inflammatory vascular endothelium is thought to play a causal role in secondary thrombus formation or microcirculatory disturbance after vessel occlusion. However, while adhesion receptors involved in platelet/matrix interactions have been extensively studied, the molecular mechanisms involved in platelet/endothelium interactions are incompletely characterized and have been mainly studied under static conditions. Using human platelets or platelets from wild type and CD47-/- mice in whole blood, we demonstrated that at low shear rate, CD47 expressed on human and mouse platelets significantly contributes to platelet adhesion on TNF- -stimulated vascular endothelial cells. Using the CD47 agonist peptide 4N1K and blocking monoclonal antibodies (mAbs), we showed that CD47 binds the CBD domain of the endothelial TSP-1 inducing the activation of the platelet IIb 3 integrin which in turn becomes able to link the endothelial receptors ICAM-1 and v3. Platelet CD36 and GPIb are involved too since, platelet incubation with blocking mAbs directed against each of these two receptors significantly decreased platelet arrest. As anti-CD47 treatment of platelets did not further decrease adhesion of anti-CD36-treated platelets and, as CD36 is a TSP-1 receptor, this suggests that CD36/TSP-1 interaction could trigger the CD47-dependent pathway. Overall, CD47 antagonists would be of significant clinical importance.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Bonnefoy, K. Daenens, H. B. Feys, R. De Vos, P. Vandervoort, J. Vermylen, J. Lawler, and M. F. Hoylaerts
Thrombospondin-1 controls vascular platelet recruitment and thrombus adherence in mice by protecting (sub)endothelial VWF from cleavage by ADAMTS13
Blood,
February 1, 2006;
107(3):
955 - 964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Tull, S. I. Anderson, S. C. Hughan, S. P. Watson, G. B. Nash, and G. E. Rainger
Cellular Pathology of Atherosclerosis: Smooth Muscle Cells Promote Adhesion of Platelets to Cocultured Endothelial Cells
Circ. Res.,
January 6, 2006;
98(1):
98 - 104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Rosenthal-Allieri, M. Ticchioni, J. P. Breittmayer, Y. Shimizu, and A. Bernard
Influence of {beta}1 Integrin Intracytoplasmic Domains in the Regulation of VLA-4-Mediated Adhesion of Human T Cells to VCAM-1 under Flow Conditions
J. Immunol.,
July 15, 2005;
175(2):
1214 - 1223.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Tabibiazar, R. A. Wagner, J. M. Spin, E. A. Ashley, B. Narasimhan, E. M. Rubin, B. Efron, P. S. Tsao, R. Tibshirani, and T. Quertermous
Mouse Strain-Specific Differences in Vascular Wall Gene Expression and Their Relationship to Vascular Disease
Arterioscler. Thromb. Vasc. Biol.,
February 1, 2005;
25(2):
302 - 308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Shattil and P. J. Newman
Integrins: dynamic scaffolds for adhesion and signaling in platelets
Blood,
September 15, 2004;
104(6):
1606 - 1615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
