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Prepublished online as a Blood First Edition Paper on April 24, 2003; DOI 10.1182/blood-2002-11-3541. This Article Full Text (PDF) All Versions of this Article: 2002-11-3541v1 102/4/1298    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nemeth, M. J Articles by Bodine, D. M Search for Related Content PubMed PubMed Citation Articles by Nemeth, M. J Articles by Bodine, D. M Social Bookmarking                   What's this? Submitted November 21, 2002 Accepted April 16, 2003 Hmgb3: an HMG-box family member expressed in primitive hematopoietic cells that inhibits myeloid and B-cell differentiation Michael J Nemeth, David J Curtis, Martha R Kirby, Lisa J Garrett-Beal, Nancy E Seidel, Amanda P Cline, and David M Bodine* Hematopoiesis Section, National Human Genome Research Institute, Bethesda, MD, USA National Human Genome Research Institute Transgenic Mouse Core, Bethesda, MD, USA Rotary Bone Marrow Research Laboratory, Royal Melbourne Hospital, Melbourne, VIC, Australia * Corresponding author; email: tedyaz{at}nhgri.nih.gov. Hmgb3 is a member of a family of chromatin-binding proteins which can alter DNA structure to facilitate transcription factor binding. We identified the Hmgb3 cDNA in a subtractive hybridization screen for transcripts that are preferentially expressed within hematopoietic stem cells. We inserted an IRES-GFP cassette into the 3' untranslated region of the X-linked Hmgb3 locus to identify Hmgb3 expressing cells. In adult mice, Hmgb3 mRNA is detected in bone marrow cells, primitive Lin-, c-kit+, Sca-1+, IL-7R- cells and Ter119+ erythroid cells. We observed that long-term repopulating ability is entirely contained in the sub-population of Lin-, c-kitHI cells that express Hmgb3. The majority of common lymphoid and myeloid progenitors express Hmgb3. Introduction of a retrovirus containing the Hmgb3 cDNA into mouse bone marrow stem cells demonstrated that enforced expression of Hmgb3 inhibited B-cell and myeloid differentiation. We conclude that down-regulation of Hmgb3 protein levels is an important step for myeloid and B-cell differentiation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Catena, E. Escoffier, C. Caron, S. Khochbin, I. Martianov, and I. Davidson HMGB4, a Novel Member of the HMGB Family, Is Preferentially Expressed in the Mouse Testis and Localizes to the Basal Pole of Elongating Spermatids Biol Reprod, February 1, 2009; 80(2): 358 - 366. [Abstract] [Full Text] [PDF] M. J. Nemeth, L. Topol, S. M. Anderson, Y. Yang, and D. M. Bodine Wnt5a inhibits canonical Wnt signaling in hematopoietic stem cells and enhances repopulation PNAS, September 25, 2007; 104(39): 15436 - 15441. [Abstract] [Full Text] [PDF] M. J. Nemeth, M. R. Kirby, and D. M. Bodine Hmgb3 regulates the balance between hematopoietic stem cell self-renewal and differentiation PNAS, September 12, 2006; 103(37): 13783 - 13788. [Abstract] [Full Text] [PDF] A. Ragab, E. C. Thompson, and A. A. Travers High Mobility Group Proteins HMGD and HMGZ Interact Genetically With the Brahma Chromatin Remodeling Complex in Drosophila Genetics, February 1, 2006; 172(2): 1069 - 1078. [Abstract] [Full Text] [PDF] A. Bulfone, P. Carotenuto, A. Faedo, V. Aglio, L. Garzia, A. M. Bello, A. Basile, A. Andre, M. Cocchia, O. Guardiola, et al. Telencephalic Embryonic Subtractive Sequences: A Unique Collection of Neurodevelopmental Genes J. Neurosci., August 17, 2005; 25(33): 7586 - 7600. [Abstract] [Full Text] [PDF] M. J. Nemeth, A. P. Cline, S. M. Anderson, L. J. Garrett-Beal, and D. M. Bodine Hmgb3 deficiency deregulates proliferation and differentiation of common lymphoid and myeloid progenitors Blood, January 15, 2005; 105(2): 627 - 634. [Abstract] [Full Text] [PDF]

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