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Prepublished online as a Blood First Edition Paper on May 8, 2003; DOI 10.1182/blood-2002-11-3551. This Article Full Text (PDF) All Versions of this Article: 2002-11-3551v1 102/5/1771    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Barry, S. M Articles by Kansas, G. S Search for Related Content PubMed PubMed Citation Articles by Barry, S. M Articles by Kansas, G. S Social Bookmarking                   What's this? Submitted November 22, 2002 Accepted April 22, 2003 Induction of FucT-VII by the Ras/MAP kinase cascade in Jurkat T cells Sheila M Barry, Dimitrios G Zisoulis, Joel H Neal, Neil A Clipstone, and Geoffrey S Kansas* Microbiology-Immunology, Northwestern University Medical School, Chicago, IL, USA * Corresponding author; email: gsk{at}northwestern.edu. Induction of the a1,3 fucosyltransferase FucT-VII in T lymphocytes is crucial for selectin ligand formation, but the signaling and transcriptional pathways which govern FucT-VII expression are unknown. Here, using a novel, highly PMA-responsive variant of the Jurkat T cell line, we identify Ras and downstream MAP kinase pathways as essential mediators of FucT-VII gene expression. PMA induced FucT-VII in only a subset of treated cells, similar to expression of FucT-VII in normal activated CD4 T cells. Introduction of constitutively active Ras or Raf by recombinant retroviruses induced FucT-VII expression only in that subset of cells expressing the highest levels of Ras, suggesting that induction of FucT-VII required a critical threshhold of Ras signaling. Both PMA treatment and introduction of active Ras led to rolling on E-selectin. Pharmacologic inhibition studies confirmed the involvement of the classic Ras-Raf-MEK-ERK pathway in FucT-VII induction by PMA, Ras, and Raf. These studies also revealed a second, Ras-induced, Raf1-independent pathway which participated in induction of FucT-VII. Strong activation of Ras represents a major pathway for induction of FucT-VII gene expression in T cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Jennrich, B. A. Ratsch, A. Hamann, and U. Syrbe Long-Term Commitment to Inflammation-Seeking Homing in CD4+ Effector Cells J. Immunol., June 15, 2007; 178(12): 8073 - 8080. [Abstract] [Full Text] [PDF] G.-Y. Chen, H. Osada, L. F. Santamaria-Babi, and R. Kannagi Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes PNAS, November 7, 2006; 103(45): 16894 - 16899. [Abstract] [Full Text] [PDF] D. M. Koelle, J. Huang, M. T. Hensel, and C. L. McClurkan Innate Immune Responses to Herpes Simplex Virus Type 2 Influence Skin Homing Molecule Expression by Memory CD4+ Lymphocytes J. Virol., March 15, 2006; 80(6): 2863 - 2872. [Abstract] [Full Text] [PDF] G. H. Underhill, D. G. Zisoulis, K. P. Kolli, L. G. Ellies, J. D. Marth, and G. S. Kansas A crucial role for T-bet in selectin ligand expression in T helper 1 (Th1) cells Blood, December 1, 2005; 106(12): 3867 - 3873. [Abstract] [Full Text] [PDF] D. A. Carlow, M. J. Williams, and H. J. Ziltener Inducing P-Selectin Ligand Formation in CD8 T Cells: IL-2 and IL-12 Are Active In Vitro but Not Required In Vivo J. Immunol., April 1, 2005; 174(7): 3959 - 3966. [Abstract] [Full Text] [PDF] D. G. Zisoulis and G. S. Kansas H-Ras and Phosphoinositide 3-Kinase Cooperate to Induce {alpha}(1,3)-Fucosyltransferase VII Expression in Jurkat T Cells J. Biol. Chem., September 17, 2004; 279(38): 39495 - 39504. [Abstract] [Full Text] [PDF]

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