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Prepublished online as a Blood First Edition Paper on March 20, 2003; DOI 10.1182/blood-2002-12-3674. This Article Full Text (PDF) All Versions of this Article: 2002-12-3674v1 102/2/477    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhou, P. Articles by Comenzo, R. L Search for Related Content PubMed PubMed Citation Articles by Zhou, P. Articles by Comenzo, R. L Social Bookmarking                   What's this? Submitted December 4, 2002 Accepted March 12, 2003 Melphalan-mobilized blood stem cell components contain minimal clonotypic myeloma cell contamination Ping Zhou, Yana Zhang, Carmen Martinez, Nagesh Kalakonda, Stephen D Nimer, and Raymond L Comenzo* Hematology Service, Division of Hematologic Oncology, Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY, USA Sloan-Kettering Institute, New York, NY, USA * Corresponding author; email: comenzor{at}mskcc.org. Optimal methods of stem cell mobilization in multiple myeloma are undefined, and contaminating clonotypic cells could contribute to disease recurrence. A phase 2 trial of intravenous melphalan (60mg/m2) and G-CSF (10ug/kg/d) for mobilization was performed. To enhance reliability, contamination was assessed with two sensitive methods, immunoglobulin light- and heavy-chain variable region patient-specific limiting-dilution PCR. We evaluated 29 stem cell components (SCC) from fifteen patients; for 9 SCC, only VL PCR was used because of light chain disease or technical problems with VH primers. For 20 SCC, VL and VH PCR results were highly correlated (r2 = 0.93, p << 0.01) with 35% (7/20) having identical estimates. VH PCR gave significantly higher estimates for 8, and VL PCR for 5, SCC, supporting the utility of employing two methods. Estimated clonotypic contamination per SCC was 0.0009% (0-0.1) or 0.5 x 104 clonotypic cells per kg (0-41.2), and contamination correlated with CD34+ cells collected (r2 = 0.42, p << 0.01). Melphalan-mobilized SCC contain minimal clonotypic contamination. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. L. Comenzo, P. Zhou, M. Fleisher, B. Clark, and J. Teruya-Feldstein Seeking confidence in the diagnosis of systemic AL (Ig light-chain) amyloidosis: patients can have both monoclonal gammopathies and hereditary amyloid proteins Blood, May 1, 2006; 107(9): 3489 - 3491. [Abstract] [Full Text] [PDF]

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