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Prepublished online as a Blood First Edition Paper on April 10, 2003; DOI 10.1182/blood-2002-12-3809. This Article Full Text (PDF) All Versions of this Article: 2002-12-3809v1 102/3/896    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Minegishi, N. Articles by Yamamoto, M. Search for Related Content PubMed PubMed Citation Articles by Minegishi, N. Articles by Yamamoto, M. Social Bookmarking                   What's this? Submitted December 17, 2002 Accepted March 28, 2003 Expression and domain-specific function of GATA-2 during differentiation of the hematopoietic precursor cells in midgestation mouse embryos Naoko Minegishi, Norio Suzuki, Tomomasa Yokomizo, Xiaoqing Pan, Tetsuhiro Fujimoto, Satoru Takahashi, Takahiko Hara, Atsushi Miyajima, Shin-ichi Nishikawa, and Masayuki Yamamoto* Institute of Basic Medical Sciences,University of Tsukuba, Tsukuba, Ibaraki, Japan; Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Ibaraki, Japan Institute of Basic Medical Sciences,University of Tsukuba, Tsukuba, Ibaraki, Japan Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan Department of Tumor Biochemistry, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan; Institute of Molecular and Cellular Biology, University of Tokyo, Tokyo, Japan Institute of Molecular and Cellular Biology, University of Tokyo, Tokyo, Japan * Corresponding author; email: masi{at}tara.tsukuba.ac.jp. The aorta-gonads-mesonephros (AGM) region of the mouse embryo has been assigned as the origin of definitive hematopoiesis. The transcription factor GATA-2 has specific but unclarified roles in early hematopoiesis. To elucidate the expression profile of GATA-2, we prepared transgenic mouse lines containing the green fluorescent protein (GFP) gene driven by GATA-2 gene regulatory elements. We also prepared a mouse line in which GFP reporter sequences were inserted into the endogenous GATA-2 gene. Both mouse mutants expressed GFP in the early hematopoieic tissues. The CD45 antigen, a marker of hematopoietic cells, was expressed in a small fraction of transgene-derived (TG)-GFP+ cells. The remaining TG-GFP+/CD45- cells were adherent to plastic and produced CD45+ hematopoietic cells abundantly when cultured in vitro. Exogenous expression of GATA-2 in TG-GFP+/CD45- cells from the AGM region inhibited their differentiation into CD45+ cells. Loss of GATA-2 function through the disruption of the GATA-2 locus enhanced the earlier emergence of CD45+ cells in the yolk sac of the 9.5-day conceptus. These results demonstrated that GATA-2 is expressed in the precursor of hematopoietic cells and works as a gatekeeper to preserve their immaturity. A reduction of GATA-2 expression or activity is required for the differentiation of precursors to hematopoietic cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Nozawa, N. Suzuki, M. Kobayashi-Osaki, X. Pan, J. D. Engel, and M. Yamamoto GATA2-dependent and region-specific regulation of Gata2 transcription in the mouse midbrain Genes Cells, May 1, 2009; 14(5): 569 - 582. [Abstract] [Full Text] [PDF] L. Pase, J. E. Layton, W. P. Kloosterman, D. Carradice, P. M. Waterhouse, and G. J. 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