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Prepublished online as a Blood First Edition Paper on March 20, 2003; DOI 10.1182/blood-2002-12-3853. This Article Full Text (PDF) All Versions of this Article: 2002-12-3853v1 102/2/689    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dziarski, R. Articles by Gupta, D. Search for Related Content PubMed PubMed Citation Articles by Dziarski, R. Articles by Gupta, D. Related Collections Related Article in Blood Online Social Bookmarking                   What's this? Submitted December 19, 2002 Accepted March 11, 2003 Defect in neutrophil killing and increased susceptibility to infection with non-pathogenic Gram-positive bacteria in peptidoglycan recognition protein-S (PGRP-S)-deficient mice Roman Dziarski*, Kenneth A Platt, Eva Gelius, Hakan Steiner, and Dipika Gupta Northwest Center for Medical Education, Indiana University School of Medicine, Gary, IN, USA Lexicon Genetics, Woodlands, TX, USA Department of Microbiology, Stockholm University, Stockholm, Sweden * Corresponding author; email: rdziar{at}iun.edu. Insect peptidoglycan recognition protein-S (PGRP-S), a member of a family of innate immunity pattern recognition molecules conserved from insects to mammals, recognizes bacterial cell wall peptidoglycan and activates two antimicrobial defense systems, prophenoloxidase cascade and antimicrobial peptides through Toll receptor. We show that mouse PGRP-S is present in neutrophil tertiary granules and that PGRP-S-deficient (PGRP-S-/-) mice have increased susceptibility to intraperitoneal infection with Gram-positive bacteria of low pathogenicity, but not with more pathogenic Gram-positive or Gram-negative bacteria. PGRP-S-/- mice have normal inflammatory responses and production of TNF- and IL-6. Neutrophils from PGRP-S-/- mice have normal phagocytic uptake of bacteria, but are defective in intracellular killing and digestion of relatively non-pathogenic Gram-positive bacteria. Therefore, mammalian PGRP-S functions in intracellular killing of bacteria. Thus, only bacterial recognition by PGRP-S, but not its effector function, is conserved from insects to mammals. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Peptidoglycan recognition and neutrophil function Jerrold Weiss Blood 2003 102: 419. 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