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Prepublished online as a Blood First Edition Paper on May 8, 2003; DOI 10.1182/blood-2002-12-3854. This Article Full Text (PDF) All Versions of this Article: 2002-12-3854v1 102/5/1753    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jefford, M. Articles by Maraskovsky, E. Search for Related Content PubMed PubMed Citation Articles by Jefford, M. Articles by Maraskovsky, E. Social Bookmarking                   What's this? Submitted December 27, 2002 Accepted April 15, 2003 Functional comparison of DC generated in vivo with Flt3 ligand or in vitro from blood monocytes: Differential regulation of function by specific classes of physiologic stimuli Michael Jefford, Max Schnurr, Tracey Toy, Kelly Anne Masterman, Amanda Shin, Tina Beecroft, Tsin Yee Tai, Ken Shortman, Mark Shackleton, Ian D Davis, Phil Parente, Thomas Luft, Weisan Chen, Jonathan Cebon, and Eugene Maraskovsky* Tumour Biology, Ludwig Institute for Cancer Research, Heidelberg, VIC, Australia Immunology, The Walter and Eliza Institute of Medical Research, Parkville, VIC, Australia Medizinische Klinik und Poliklinik V, University of Heidelberg, Heidelberg, Germany * Corresponding author; email: eugene.maraskovsky{at}ludwig.edu.au. Dendritic cells (DC) are a family of leukocytes that initiate T and B cell immunity against pathogens. Migration of antigen-loaded DC from sites of infection into draining lymphoid tissues is fundamental to the priming of T cell immune responses. In humans, the major peripheral blood DC (PBDC) types, CD1b/c+ DC and IL-3R+ plasmacytoid DC, are significantly expanded in vivo using Flt3 ligand (FL). DC-like cells can also be generated from monocyte precursors (MoDC). A detailed comparison of the functional potential of these types of DC (in an autologous setting) has yet to be reported. Here, we compared the functional capacity of FL-expanded CD1b/c+ PBDC with autologous MoDC in response to three different classes of stimuli: (1) pro-inflammatory mediators, (2) soluble CD40L (CD40L) and (3) intact bacteria (E.coli). Significant differences in functional capacities were found with respect to changes in phenotype, migratory capacity, cytokine secretion and T cell stimulation. MoDC required specific stimuli for the expression of functions. They responded vigorously to CD40L or E.coli, expressing cytokines known to regulate IFN- in T cells (IL-12p70, IL-18 and IL-23) but required PGE2 during stimulation to migrate to chemokines. In contrast, PBDC matured in response to minimal stimulation, rapidly acquired migratory function in the absence of PGE2-containing stimuli and were low cytokine producers. Interestingly, both types of DC were equivalent with respect to stimulation of allogeneic-T cell proliferation and presentation of peptides to CTL lines. These distinct differences are of particular importance when considering the choice of DC types for clinical applications. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. L. Wong, L. F. M. Tang, F. C. Lew, H. S. K. Wong, Y. L. Chua, P. A. MacAry, and D. M. Kemeny CD44high Memory CD8 T Cells Synergize with CpG DNA to Activate Dendritic Cell IL-12p70 Production J. 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