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Prepublished online as a Blood First Edition Paper on June 5, 2003; DOI 10.1182/blood-2002-12-3921. This Article Full Text (PDF) All Versions of this Article: 2002-12-3921v1 102/7/2506    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cohen-Saidon, C. Articles by Razin, E. Search for Related Content PubMed PubMed Citation Articles by Cohen-Saidon, C. Articles by Razin, E. Social Bookmarking                   What's this? Submitted December 26, 2002 Accepted May 19, 2003 A novel strategy using single chain antibody to show the importance of Bcl-2 in mast cell survival Cellina Cohen-Saidon, Hovav Nechushtan, Shira Kahlon, Nadav Livni, Ahuva Nissim, and Ehud Razin* Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem, Israel Sackler Faculty of Medicine, Felsenstein Medical Research Center, Tel Aviv, Israel Bone and Joint Research Unit, Bart and the London, Queen Mary's School of Medicine and Dentistry, London, United Kingdom * Corresponding author; email: ehudr{at}cc.huji.ac.il. Apoptosis or programmed cell death plays an important role in a wide variety of physiological processes and is regulated by proteins of the Bcl-2 family consisting of both anti-apoptotic and pro-apoptotic factors. The direct involvement of the Bcl-2 protein family in the process of mast cell apoptosis has not been clarified. In the present work we have used a single-chain antibody (scFv) raised against Bcl-2 derived from a semi-synthetic human phage-display antibody library. The addition of TAT sequence, which is responsible for translocation through the membrane, endows the anti-Bcl-2-scFv with the ability to penetrate living cells. Moreover, it specifically neutralizes Bcl-2 intracellularly by binding to the BH1 domain and eradicates its anti-apoptotic activity in two types of mast cells and in a human breast cancer cell line. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Avignolo, L. Bagnasco, B. Biasotti, A. Melchiori, V. Tomati, I. Bauer, A. Salis, L. Chiossone, M. C. Mingari, P. Orecchia, et al. Internalization via Antennapedia protein transduction domain of an scFv antibody toward c-Myc protein FASEB J, April 1, 2008; 22(4): 1237 - 1245. [Abstract] [Full Text] [PDF] M. Kovaleva, I. Bussmeyer, B. Rabe, J. Grotzinger, E. Sudarman, J. Eichler, U. Conrad, S. Rose-John, and J. Scheller Abrogation of Viral Interleukin-6 (vIL-6)-Induced Signaling by Intracellular Retention and Neutralization of vIL-6 with an Anti-vIL-6 Single-Chain Antibody Selected by Phage Display. J. Virol., September 1, 2006; 80(17): 8510 - 8520. [Abstract] [Full Text] [PDF] C. Cohen-Saidon, I. Carmi, A. Keren, and E. Razin Antiapoptotic function of Bcl-2 in mast cells is dependent on its association with heat shock protein 90beta Blood, February 15, 2006; 107(4): 1413 - 1420. [Abstract] [Full Text] [PDF] A. Hachem and R. B. Gartenhaus Oncogenes as molecular targets in lymphoma Blood, September 15, 2005; 106(6): 1911 - 1923. [Full Text] [PDF] C. Moller, J. Alfredsson, M. Engstrom, H. Wootz, Z. Xiang, J. Lennartsson, J.-I. Jonsson, and G. Nilsson Stem cell factor promotes mast cell survival via inactivation of FOXO3a-mediated transcriptional induction and MEK-regulated phosphorylation of the proapoptotic protein Bim Blood, August 15, 2005; 106(4): 1330 - 1336. [Abstract] [Full Text] [PDF]

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