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Prepublished online as a Blood First Edition Paper on June 19, 2003; DOI 10.1182/blood-2003-01-0007. This Article Full Text (PDF) All Versions of this Article: 2003-01-0007v1 102/8/2724    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Meissner, A. Articles by Korner, H. Search for Related Content PubMed PubMed Citation Articles by Meissner, A. Articles by Korner, H. Social Bookmarking                   What's this? Submitted January 2, 2003 Accepted June 6, 2003 CC-chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress Anja Meissner, Olaf Zilles, Rosa Varona, Katrin Jozefowski, Uwe Ritter, Gabriel Marquez, Rupert Hallmann, and Heinrich Korner* IZKF NW1, Nikolaus-Fiebiger-Center for Molecular Medicine, Erlangen, Germany Department for Experimental Medicine I, Nikolaus-Fiebiger-Center for Molecular Medicine, Erlangen, Germany; Department of Experimental Pathology, Lund University, Lund, Sweden Departamento de Immunologia y Oncologia, Universidad Autonoma, Madrid, Spain Department of Experimental Pathology, Lund University, Lund, Sweden; Department for Experimental Medicine I, Nikolaus-Fiebiger-Center for Molecular Medicine, Erlangen, Germany * Corresponding author; email: hkoerner{at}molmed.uni-erlangen.de. Chemokines are thought to control lymphocyte recruitment to the inflamed endothelium. To dissect chemokine-mediated adhesion, binding of ex vivo isolated splenocytes to TNF-activated endothelial cells was analyzed under shear stress. We observed specific adhesion of naive follicular B cells, which could be blocked by pertussis toxin. This indicated a G-protein mediated binding and pointed at a contribution of chemokine receptors to B cell-adhesion. Analysis of chemokines expressed by TNF-activated endothelial cells showed that CC-chemokine-ligand 2 (CCL2), CCL17 and CCL20 were upregulated. Only on follicular B cells the cognate receptor for CCL20, CC-chemokine receptor 6 (CCR6), was expressed strongly and a functional transmigration assay with CCR6-negative B cells demonstrated conclusively the sole signaling of CCL20 through CCR6. Desensitization of CCR6 on naive B cells with CCL20 resulted in receptor downregulation and reduced B cell adhesion. We conclude that CCL20 plays a vital role in B cell adhesion to the inflamed endothelium. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. K. Lundy, S. A. Lira, J. J. Smit, D. N. Cook, A. A. Berlin, and N. W. Lukacs Attenuation of Allergen-Induced Responses in CCR6-/- Mice Is Dependent upon Altered Pulmonary T Lymphocyte Activation J. Immunol., February 15, 2005; 174(4): 2054 - 2060. [Abstract] [Full Text] [PDF] U. Ritter, F. Wiede, D. Mielenz, Z. Kiafard, J. Zwirner, and H. Korner Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells J. Leukoc. Biol., August 1, 2004; 76(2): 472 - 476. [Abstract] [Full Text] [PDF]

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