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Prepublished online as a Blood First Edition Paper on June 26, 2003; DOI 10.1182/blood-2003-01-0045. This Article Full Text (PDF) All Versions of this Article: 2003-01-0045v1 102/8/2994    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sanchez, M.-L. Articles by Orfao, A. Search for Related Content PubMed PubMed Citation Articles by Sanchez, M.-L. Articles by Orfao, A. Social Bookmarking                   What's this? Submitted January 7, 2003 Accepted June 10, 2003 Incidence and clinico-biologic characteristics of leukemic B-cell chronic lymphoproliferative disorders with more than one B-cell clone Maria-Luz Sanchez, Julia Almeida, David Gonzalez, Marcos Gonzalez, Maria-Antonia Garcia-Marcos, Ana Balanzategui, Maria-Consuelo Lopez-Berges, Josep Nomdedeu, Teresa Vallespi, Marcos Barbon, Alejandro Martin, Pilar de la Fuente, Guillermo Martin-Nunez, Javier Fernandez-Calvo, Jesus-Maria Hernandez, Jesus F San-Miguel, and Alberto Orfao* Servicio de Citometria y Departamento de Medicina, Universidad de Salamanca, Salamanca, Spain; Centro de Investigacion del Cancer, Universidad de Salamanca, Salamanca, Spain Servicio de Hematologia, Hospital Universitario de Salamanca, Salamanca, Spain Centro de Investigacion del Cancer, Universidad de Salamanca, Salamanca, Spain; Servicio de Hematologia, Hospital Universitario de Salamanca, Salamanca, Spain Servicio de Hematologia, Hospital Santa Creu i Sant Pau, Barcelona, Spain Servicio de Hematologia, Hospital Valle del Hebron, Barcelona, Spain Servicio de Hematologia, Hospital Virgen Blanca, Leon, Spain Servicio de Hematologia, Hospital Virgen de la Concha, Zamora, Spain Servicio de Hematologia, Hospital General Yague, Burgos, Spain Servicio de Hematologia, Hospital Virgen del Puerto, Plasencia, Caceres, Spain Servicio de Hematologia, Hospital Clinico de Valladolid, Valladolid, Spain * Corresponding author; email: orfao{at}usal.es. Leukemic B-chronic lymphoproliferative disorders (B-CLPD) are generally believed to derive from a monoclonal B-cell; biclonality has only occasionally been reported. In this study, we have explored the incidence of B-CLPD cases with 2 B-cell clones, and established both the phenotypic differences between the coexisting clones and the clinico-biologic features of these patients. In total, 53 B-CLPD cases with 2 B-cell clones were studied. Presence of 2 B-cell clones was suspected by immunophenotype and confirmed by molecular/genetic techniques in leukemic samples (n=42) and purified B-cell subpopulations (n=10). Overall, 4.8% of 477 consecutive B-CLPD had 2 B-cell clones, their incidence being specially higher among hairy cell leukemia (3/13), large cell lymphoma (2/10) and atypical chronic lymphocytic leukemia (CLL) (4/29). In most cases the two B-cell subsets displayed either different sIg light-chain (n=37/53) or different levels of the same sIg (n=9/53), usually associated with other phenotypic differences. As compared to monoclonal cases, B-CLL patients with 2 clones had lower WBC and lymphocyte counts, more frequently displayed splenomegaly and required early treatment. Among these, the cases where a CLL clone coexisted with a non-CLL clone were older, and more often displayed B symptoms, monoclonal component and diffuse infiltration of bone marrow, and required early treatment more frequently than cases with monoclonal CLL or two CLL clones. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. A. Hernandez, A. E. Rodriguez, M. Gonzalez, R. Benito, C. Fontanillo, V. Sandoval, M. Romero, G. Martin-Nunez, A. G. de Coca, R. Fisac, et al. A high number of losses in 13q14 chromosome band is associated with a worse outcome and biological differences in patients with B-cell chronic lymphoid leukemia Haematologica, March 1, 2009; 94(3): 364 - 371. [Abstract] [Full Text] [PDF] A. C. Rawstron, A. Orfao, M. Beksac, L. Bezdickova, R. A. Brooimans, H. Bumbea, K. Dalva, G. Fuhler, J. Gratama, D. Hose, et al. Report of the European Myeloma Network on multiparametric flow cytometry in multiple myeloma and related disorders Haematologica, March 1, 2008; 93(3): 431 - 438. [Abstract] [Full Text] [PDF] J. Kriangkum, B. J. Taylor, S. P. Treon, M. J. Mant, T. Reiman, A. R. Belch, and L. M. Pilarski Molecular Characterization of Waldenstrom's Macroglobulinemia Reveals Frequent Occurrence of Two B-Cell Clones Having Distinct IgH VDJ Sequences Clin. Cancer Res., April 1, 2007; 13(7): 2005 - 2013. [Abstract] [Full Text] [PDF] R. 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