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Prepublished online as a Blood First Edition Paper on June 26, 2003; DOI 10.1182/blood-2003-01-0150. This Article Full Text (PDF) All Versions of this Article: 2003-01-0150v1 102/8/2892    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rezvani, K. Articles by Barrett, A J Search for Related Content PubMed PubMed Citation Articles by Rezvani, K. Articles by Barrett, A J Related Collections Related Letter in Blood Online Social Bookmarking                   What's this? Submitted January 17, 2003 Accepted June 12, 2003 Functional leukemia-associated antigen-specific memory CD8+ T cells exist in healthy individuals and in patients with chronic myelogenous leukemia before and after stem cell transplantation Katayoun Rezvani*, Matthias Grube, Jason M Brenchley, Giuseppe Sconocchia, Hiroshi Fujiwara, David A Price, Emma Gostick, Ko Yamada, Jan Melenhorst, Richard Childs, Nancy Hensel, Daniel C Douek, and A J Barrett Hematology Branch, NHLBI, National Institutes of Health, Bethesda, MD, USA Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, USA Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, United Kingdom * Corresponding author; email: rezvanik{at}nhlbi.nih.gov. Antigens implicated in the graft-versus-leukemia (GVL) effect in chronic myeloid leukemia (CML) include WT1, PR1 and BCR-ABL. To detect very low frequencies of these antigen-specific CD8+ T cells, we used quantitative PCR (qPCR) to measure interferon-gamma (IFN-) mRNA production by peptide-pulsed CD8+ T cells from HLA-A*0201+ healthy volunteers and CML patients before and after allogeneic stem cell transplantation (SCT). Parallel assays using CMV pp65 tetramers demonstrated the IFN- copy number to be linearly related to the frequency of tetramer-binding T cells, sensitive to frequencies of 1 responding CD8+ T cell/100,000 CD8+ T cells. Responses to WT1 and PR1 but not BCR-ABL were detected in 10/18 healthy donors. Responses to WT1, PR1 or BCR-ABL were observed in 9/14 CML patients pre-SCT and 5/6 post SCT, often to multiple epitopes. Responses were higher in CML patients compared to healthy donors and highest after SCT. These antigen specific CD8+ T cells comprised central memory (CD45RO+CD27+CD57-) and effector memory(CD45RO-CD27-CD57+) T cells. In conclusion, leukemia reactive CD8+ T cells derive from memory T cells and occur at low frequencies in healthy individuals and at higher frequencies in CML patients. The increased response in patients post-SCT suggests a quantitative explanation for the greater effect of allogeneic SCT. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letter in Blood Online: BCR-ABL–specific T cells can be detected in healthy donors and in chronic myeloid leukemia patients following allogeneic stem cell transplantation Nauman M. Butt, Lihui Wang, Hazem M. Abu-Eisha, Steve E. Christmas, and Richard E. Clark Blood 2004 103: 3245. [Full Text] [PDF] This article has been cited by other articles: C. Chaise, S. L. Buchan, J. Rice, J. Marquet, H. Rouard, M. Kuentz, G. E. Vittes, V. Molinier-Frenkel, J.-P. Farcet, H. J. Stauss, et al. 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