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Prepublished online as a Blood First Edition Paper on August 28, 2003; DOI 10.1182/blood-2003-01-0159. This Article Full Text (PDF) All Versions of this Article: 2003-01-0159v1 103/3/828    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gery, S. Articles by Koeffler, H P. Search for Related Content PubMed PubMed Citation Articles by Gery, S. Articles by Koeffler, H P. Social Bookmarking                   What's this? Submitted January 17, 2003 Accepted August 21, 2003 C/EBP interacts with Retinoblastoma and E2F1 during granulopoiesis Sigal Gery*, Adrian F Gombart, Yuen K Fung, and H Phillip Koeffler Division of Hematology/Oncology, Cedars-Sinai Medical Center, Los Angeles, CA, USA Division of Hematology/Oncology, Childrens Hospital, Los Angeles, CA, USA * Corresponding author; email: gerys{at}cshs.org. CCAAT enhancer binding protein epsilon (C/EBP) is a myeloid specific transcription factor that is essential for terminal granulocytic differentiation. Retinoblastoma (Rb) and E2F1 are critical cell-cycle regulators that have also been implicated in several differentiation systems. Here, we demonstrate that C/EBP interacts with Rb and E2F1 during granulocytic differentiation in NB4 and U937 human myeloid cells and in 32Dcl3 murine myeloid precursor cells. The interaction between C/EBP and Rb enhances C/EBP-mediated transcription of myeloid specific genes both in reporter assays and endogenously. The C/EBP-E2F1 interaction results in repression of E2F1-mediated transcriptional activity. Finally, overexpression of C/EBP in human myeloid cells leads to downregulation of c-Myc. We propose that the interactions between C/EBP, a tissue-specific transcription factor, and the broad-spectrum proteins, Rb and E2F1, are important in C/EBP induced terminal granulocytic differentiation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Bedi, J. Du, A. K. Sharma, I. Gomes, and S. J. Ackerman Human C/EBP-{epsilon} activator and repressor isoforms differentially reprogram myeloid lineage commitment and differentiation Blood, January 8, 2009; 113(2): 317 - 327. [Abstract] [Full Text] [PDF] B. J. Chyla, I. Moreno-Miralles, M. A. Steapleton, M. A. Thompson, S. Bhaskara, M. Engel, and S. W. Hiebert Deletion of Mtg16, a Target of t(16;21), Alters Hematopoietic Progenitor Cell Proliferation and Lineage Allocation Mol. Cell. Biol., October 15, 2008; 28(20): 6234 - 6247. [Abstract] [Full Text] [PDF] M. Verykokakis, C. Papadaki, E. Vorgia, L. Le Gallic, and G. Mavrothalassitis The RAS-dependent ERF Control of Cell Proliferation and Differentiation Is Mediated by c-Myc Repression J. Biol. Chem., October 12, 2007; 282(41): 30285 - 30294. [Abstract] [Full Text] [PDF] H. Wang, B. Larris, T. H. Peiris, L. Zhang, J. Le Lay, Y. Gao, and L. E. Greenbaum C/EBPbeta Activates E2F-regulated Genes in Vivo via Recruitment of the Coactivator CREB-binding Protein/P300 J. Biol. Chem., August 24, 2007; 282(34): 24679 - 24688. [Abstract] [Full Text] [PDF] A. M. Chumakov, A. Silla, E. A. Williamson, and H. P. Koeffler Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with NFkappaB pathway Blood, May 15, 2007; 109(10): 4209 - 4219. [Abstract] [Full Text] [PDF] H. Nakajima, N. Watanabe, F. Shibata, T. Kitamura, Y. Ikeda, and M. Handa N-terminal Region of CCAAT/Enhancer-binding Protein {epsilon} Is Critical for Cell Cycle Arrest, Apoptosis, and Functional Maturation during Myeloid Differentiation J. Biol. Chem., May 19, 2006; 281(20): 14494 - 14502. [Abstract] [Full Text] [PDF] P. F. Johnson Molecular stop signs: regulation of cell-cycle arrest by C/EBP transcription factors J. Cell Sci., June 15, 2005; 118(12): 2545 - 2555. [Abstract] [Full Text] [PDF] T. Ikezoe, S. Gery, D. Yin, J. O'Kelly, L. Binderup, N. Lemp, H. Taguchi, and H. P. Koeffler CCAAT/Enhancer-Binding Protein {delta}: A Molecular Target of 1,25-Dihydroxyvitamin D3 in Androgen-Responsive Prostate Cancer LNCaP Cells Cancer Res., June 1, 2005; 65(11): 4762 - 4768. [Abstract] [Full Text] [PDF]

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