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Prepublished online as a Blood First Edition Paper on May 8, 2003; DOI 10.1182/blood-2003-01-0224. This Article Full Text (PDF) All Versions of this Article: 2003-01-0224v1 102/5/1649    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by de Pooter, R. F Articles by Zuniga-Pflucker, J. C. Search for Related Content PubMed PubMed Citation Articles by de Pooter, R. F Articles by Zuniga-Pflucker, J. C. Social Bookmarking                   What's this? Submitted January 24, 2003 Accepted April 26, 2003 In vitro generation of T lymphocytes from embryonic stem cell-derived pre-hematopoietic progenitors Renee F de Pooter, Sarah K Cho, James R Carlyle, and Juan Carlos Zuniga-Pflucker* Department of Immunology, University of Toronto, Toronto, Ontario, Canada * Corresponding author; email: jc.zuniga.pflucker{at}utoronto.ca. Embryonic stem (ES) cells can differentiate into most blood cells in vitro, providing a powerful model system to study hematopoiesis. However, ES cell-derived T lymphocytes have not been generated in vitro, and it was unresolved whether such potential is absent or merely difficult to isolate. As the latter case might result from rapid commitment to non-T cell fates, we isolated ES cell-derived pre-hematopoietic precursors for reconstitution of fetal thymic organ cultures. We found a transient Flk1+ CD45- subset of these precursors generated T lymphocytes in vitro, and the use of reaggregate thymic organ cultures greatly enhanced reconstitution frequency. These findings reveal that ES cells can exhibit in vitro T cell potential, but this is restricted to early stages of ES cell differentiation. Moreover, the results support the notion that the thymic microenvironment can induce T cell differentiation from a subset of pre-hematopoietic progenitors, and suggest deficient migration into intact thymi hindered previous attempts to generate T cells in vitro from ES-cell derived progenitors. These findings demonstrate that a defined subset of ES cells has the potential to generate T cells in vitro, and could contribute to greater understanding of the molecular events of hematopoietic induction and T cell lineage commitment. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. S. 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