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Prepublished online as a Blood First Edition Paper on June 12, 2003; DOI 10.1182/blood-2003-02-0345. This Article Full Text (PDF) All Versions of this Article: 2003-02-0345v1 102/7/2498    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Papanicolaou, G. A Articles by Sadelain, M. Search for Related Content PubMed PubMed Citation Articles by Papanicolaou, G. A Articles by Sadelain, M. Social Bookmarking                   What's this? Submitted February 7, 2003 Accepted May 7, 2003 Rapid expansion of cytomegalovirus-specific cytotoxic T-lymphocytes by artificial antigen presenting cells expressing a single HLA allele Genovefa A Papanicolaou, Jean-Baptiste Latouche, Cuiwen Tan, Jakob Dupont, Jeffrey Stiles, Eric G Pamer, and Michel Sadelain* * Corresponding author; email: sadelain-m{at}ski.org. Cytomegalovirus (CMV) is a major threat in patients undergoing allogeneic bone marrow transplantation. The adoptive transfer of CMV-specific cytotoxic T lymphocytes (CTLs) expanded from the blood of CMV-seropositive donors has been shown to effectively control CMV infection. However, the requirement for safe and effective antigen-presenting cells (APCs) for each patient precludes broad applicability of this successful form of therapy. Here we analyze the ability of artificial APCs (AAPCs) to activate and expand CMV-specific CTLs from peripheral blood of seropositive HLA A2.1+ donors. We demonstrate that AAPCs expressing the CMV P495 peptide or the full-length pp65 protein stimulate P495-specific CTLs at least as effectively as autologous, peptide-pulsed, peripheral blood mononuclear cells or EBV-transformed B-cells. Starting from 100ml of blood, the AAPCs reliably yield clinically relevant CTL numbers after a single stimulation. CTLs activated on AAPCs effectively kill CMV-infected fibroblasts, and have a Tc1 memory effector phenotype, identical to that of CTLs generated with autologous APCs. AAPCs thus offer a rapid, controlled, convenient and highly reproducible system for expaniding CMV-specific CTLs. Furthermore, the CTL expansion obtained with AAPCs encoding full length pp65 indicate that AAPCs may be used to present known as well as unknown CTL epitopes in the context of the AAPC's HLA. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. N. Hasan, W. J. Kollen, D. Trivedi, A. 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