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Blood, 1 May 2004, Vol. 103, No. 9, pp. 3320-3325. Prepublished online as a Blood First Edition Paper on December 30, 2003; DOI 10.1182/blood-2003-02-0352. This Article Full Text (PDF) All Versions of this Article: 2003-02-0352v1 103/9/3320    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Koibuchi, N. Articles by Morishita, R. Search for Related Content PubMed PubMed Citation Articles by Koibuchi, N. Articles by Morishita, R. Social Bookmarking                   What's this? Submitted February 3, 2003 Accepted December 19, 2003 Essential role of HGF (hepatocyte growth factor) in blood formation in Xenopus Nobutaka Koibuchi, Yasufumi Kaneda, Yoshiaki Taniyama, Kunio Matsumoto, Toshikazu Nakamura, Toshio Ogihara, and Ryuichi Morishita* Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan; Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan Division of Biochemistry, Department of Oncology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan * Corresponding author; email: morishit{at}cgt.med.osaka-u.ac.jp. In this study, we investigated the role of hepatocyte growth factor (HGF) in blood formation during Xenopus development. First, we examined the gene expression of HGF and its receptor, c-met, by whole-mount in situ hybridization during development. Strong signals of HGF as well as c-met were detected early in the developing ventral mesoderm which later gives rise to the ventral blood islands. Furthermore, to study the role of HGF, we blocked HGF signaling pathway in Xenopus embryos by using truncated c-met lacking the tyrosine kinase domain. Injection of truncated c-met mRNA resulted in a marked decrease in the number of circulating blood cells. Similar results were obtained using morpholino antisense HGF oligonucleotides. Moreover, we also analyzed the expression of several early primitive blood markers in the blood islands of these embryos. RNA in situ analysis revealed a significant reduction (or absence) of SCL, -globin and GATA-1 expression, but not GATA-2 expression. In contrast, no significant difference was observed in the levels of expression of early definitive blood markers, SCL, GATA-2 and GATA-3 in the dorsolateral plate, as analyzed by in situ hybridization. Overall, the present study demonstrated that HGF is necessary for primitive hematopoiesis by regulating the expression of SCL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Hayashi, H. Nakagami, Y. Takami, H. Koriyama, M. Mori, K. Tamai, J. Sun, K. Nagao, R. Morishita, and Y. Kaneda FHL-2 Suppresses VEGF-Induced Phosphatidylinositol 3-Kinase/Akt Activation via Interaction With Sphingosine Kinase-1 Arterioscler Thromb Vasc Biol, June 1, 2009; 29(6): 909 - 914. [Abstract] [Full Text] [PDF] K. Mood, C. Saucier, Y.-S. Bong, H.-S. Lee, M. Park, and I. O. Daar Gab1 Is Required for Cell Cycle Transition, Cell Proliferation, and Transformation Induced by an Oncogenic Met Receptor Mol. Biol. Cell, September 1, 2006; 17(9): 3717 - 3728. [Abstract] [Full Text] [PDF]

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