SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on July 31, 2003; DOI 10.1182/blood-2003-02-0496. This Article Full Text (PDF) All Versions of this Article: 2003-02-0496v1 102/10/3637    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Thai, L. M Articles by Jackson, D. E Search for Related Content PubMed PubMed Citation Articles by Thai, L. M Articles by Jackson, D. E Social Bookmarking                   What's this? Submitted February 20, 2003 Accepted July 21, 2003 Physical proximity and functional interplay of PECAM-1 with the Fc receptor FcRIIA on the platelet plasma membrane Le M Thai, Leonie K Ashman, Stacey N Harbour, P Mark Hogarth, and Denise E Jackson* Austin Research Institute, Austin Hospital, Melbourne, VIC, Australia University of Newcastle, Callaghan, NSW, Australia * Corresponding author; email: d.jackson{at}ari.unimelb.edu.au. We and others have recently defined that Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1/CD31) functions as a negative regulator of platelet-collagen interactions involving GPVI/FcR- chain signaling pathway. In this study, we hypothesized that PECAM-1 may be physically and functionally associated with FcRIIa on the platelet membrane. The functional relationship between PECAM-1 and FcRIIa was assessed by determining the effect of anti-PECAM-1 monoclonal antibody Fab fragments on FcRIIa-mediated platelet aggregation and heparin-induced thrombocytopenia (HITS)-mediated platelet aggregation. Preincubation of washed platelets with monoclonal antibody fragments of 2BD4 directed against PECAM-1 and IV.3 directed against FcRIIa completely blocked FcRIIa-mediated platelet aggregation and HITS-mediated platelet aggregation, while anti-CD151 antibody had no blocking effect. Co-engagement of FcRIIa and PECAM-1 resulted in negative regulation of FcRIIa-mediated phospholipase C2 activation, calcium mobilization and phosphoinositide 3-kinase-dependent signalling pathways. In addition, the physical proximity of FcRIIa and PECAM-1 was confirmed using fluorescence resonance energy transfer and co-immunoprecipitation studies. These results indicate that PECAM-1 and FcRIIa are co-localized on the platelet membrane and PECAM-1 down-regulates FcRIIa-mediated platelet responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. H. Serezani, D. M. Aronoff, R. G. Sitrin, and M. Peters-Golden Fc{gamma}RI ligation leads to a complex with BLT1 in lipid rafts that enhances rat lung macrophage antimicrobial functions Blood, October 8, 2009; 114(15): 3316 - 3324. [Abstract] [Full Text] [PDF] J. Rivera, M. L. Lozano, L. Navarro-Nunez, and V. Vicente Platelet receptors and signaling in the dynamics of thrombus formation Haematologica, May 1, 2009; 94(5): 700 - 711. [Abstract] [Full Text] [PDF] R. K. Andrews, D. Karunakaran, E. E. Gardiner, and M. C. Berndt Platelet Receptor Proteolysis: A Mechanism for Downregulating Platelet Reactivity Arterioscler Thromb Vasc Biol, July 1, 2007; 27(7): 1511 - 1520. [Abstract] [Full Text] [PDF] F. A. Lee, M. van Lier, I. A. M. Relou, L. Foley, J.-W. N. Akkerman, H. F. G. Heijnen, and R. W. Farndale Lipid Rafts Facilitate the Interaction of PECAM-1 with the Glycoprotein VI-FcR {gamma}-Chain Complex in Human Platelets J. Biol. Chem., December 22, 2006; 281(51): 39330 - 39338. [Abstract] [Full Text] [PDF] M. W. Goschnick, L.-M. Lau, J. L. Wee, Y. S. Liu, P. M. Hogarth, L. M. Robb, M. J. Hickey, M. D. Wright, and D. E. Jackson Impaired "outside-in" integrin {alpha}IIbbeta3 signaling and thrombus stability in TSSC6-deficient mice Blood, September 15, 2006; 108(6): 1911 - 1918. [Abstract] [Full Text] [PDF] S. Falati, S. Patil, P. L. Gross, M. Stapleton, G. Merrill-Skoloff, N. E. Barrett, K. L. Pixton, H. Weiler, B. Cooley, D. K. Newman, et al. Platelet PECAM-1 inhibits thrombus formation in vivo Blood, January 15, 2006; 107(2): 535 - 541. [Abstract] [Full Text] [PDF] J. L. Wee and D. E. Jackson The Ig-ITIM superfamily member PECAM-1 regulates the "outside-in" signaling properties of integrin {alpha}IIb{beta}3 in platelets Blood, December 1, 2005; 106(12): 3816 - 3823. [Abstract] [Full Text] [PDF] L.-M. Lau, J. L. Wee, M. D. Wright, G. W. Moseley, P. M. Hogarth, L. K. Ashman, and D. E. Jackson The tetraspanin superfamily member CD151 regulates outside-in integrin {alpha}IIb{beta}3 signaling and platelet function Blood, October 15, 2004; 104(8): 2368 - 2375. [Abstract] [Full Text] [PDF] S. M. Albelda, K. C. Lau, P. Chien, Z.-Y. Huang, E. Arguiris, A. Bohen, J. Sun, J. A. Billet, M. Christofidou-Solomidou, Z. K. Indik, et al. Role for Platelet-Endothelial Cell Adhesion Molecule-1 in Macrophage Fc{gamma} Receptor Function Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2): 246 - 255. [Abstract] [Full Text] [PDF] J. M. Gibbins Platelet adhesion signalling and the regulation of thrombus formation J. Cell Sci., July 15, 2004; 117(16): 3415 - 3425. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020