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Prepublished online as a Blood First Edition Paper on September 22, 2003; DOI 10.1182/blood-2003-02-0587. This Article Full Text (PDF) All Versions of this Article: 2003-02-0587v1 103/3/1147    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Grewal, S. S Articles by Wagner, J. E Search for Related Content PubMed PubMed Citation Articles by Grewal, S. S Articles by Wagner, J. E Related Collections Related Article in Blood Online Social Bookmarking                   What's this? Submitted February 25, 2003 Accepted September 11, 2003 Successful hematopoietic stem cell transplantation for Fanconi anemia from an unaffected HLA genotypically-identical sibling selected using preimplantation genetic diagnosis Satkiran S Grewal, Jeffrey P Kahn, Margaret L MacMillan, Norma K C Ramsay, and John E Wagner* Department of Pediatric Blood and Marrow Transplantation, University of Minnesota, Minneapolis, MN, USA Center for Bioethics, University of Minnesota, Minneapolis, MN, USA * Corresponding author; email: wagne002{at}umn.edu. The only proven cure for Fanconi anemia (FA) associated bone marrow failure is successful allogeneic hematopoietic stem cell transplantation (HSCT). However, HSCT using donors other than HLA-identical siblings are associated with high morbidity and poor survival. We therefore used preimplantation genetic diagnosis (PGD) to select an embryo produced by in vitro fertilization (IVF) that was both unaffected by FA and HLA-identical with the proband. The patient was a 6-year-old female with FA and myelodysplasia previously treated with oxymetholone and prednisone. After 5 cycles of IVF with intrauterine transfer of 7 embryos over a span of 4 years, successful pregnancy ensued. Twenty-eight days after delivery, the patient was transplanted with the donor's HLA-identical umbilical cord blood hematopoietic stem cells (HSCs). Neutrophil recovery occurred on day 17 without subsequent acute or chronic graft-versus-host disease. Currently, 2.5 years after transplant, the patient is well with normal hematopoiesis. In summary, we have described the first successful transplant using HSCs from a donor selected on the basis of specific desirable disease- and HLA-characteristics using IVF and PGD. The medical, legal and ethical issues involved with this approach are discussed. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: No donor? Then create one! Richard E. Harris Blood 2004 103: 757-758. [Full Text] [PDF] This article has been cited by other articles: M. Geens, I. Mateizel, K. Sermon, M. De Rycke, C. Spits, G. Cauffman, P. Devroey, H. Tournaye, I. Liebaers, and H. Van de Velde Human embryonic stem cell lines derived from single blastomeres of two 4-cell stage embryos Hum. Reprod., November 1, 2009; 24(11): 2709 - 2717. [Abstract] [Full Text] [PDF] G N Samuel, K A Strong, I Kerridge, C F C Jordens, R A Ankeny, and P J Shaw Establishing the role of pre-implantation genetic diagnosis with human leucocyte antigen typing: what place do "saviour siblings" have in paediatric transplantation? Arch. Dis. Child., April 1, 2009; 94(4): 317 - 320. [Abstract] [Full Text] [PDF] H. Van de Velde, M. De Rycke, C. De Man, K. De Hauwere, F. Fiorentino, S. Kahraman, G. Pennings, W. Verpoest, P. Devroey, and I. Liebaers The experience of two European preimplantation genetic diagnosis centres on human leukocyte antigen typing Hum. 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