|
|
Prepublished online as a Blood First Edition Paper on July 17, 2003June 5, 2003; DOI 10.1182/blood-2003-02-0634.
Submitted February 28, 2003
Accepted May 26, 2003
The homeoprotein Hex is required for hemangioblast differentiation
Ying Guo, Rebecca Chan, Heather Ramsey, Weimin Li, Xiaodong Xie, William C Shelley, Juan Pedro Martinez-Barbera, Bernardo Bort, Kenneth Zaret, Mervin Yoder, and Robert Hromas*
Medicine and the Walther Oncology Center, Indiana University Cancer Center, Indianapolis, IN, USA
Pediatrics and the Wells Center for Pediatric Research, Indiana University Medical Center, Indianapolis, IN, USA
Cancer Reserch and Treatment Center, University of New Mexico, Albuquerque, NM, USA
Neural Development Unit, Institute of Child Health, London, United Kingdom
Cell and Developmental Biology Program, Fox Chase Cancer Center, Philadelphia, PA, USA
* Corresponding author; email: rhromas{at}salud.unm.edu.
The first hematopoietic and endothelial progenitors are derived from a common embryonic precursor termed the hemangioblast. The genetic cascades that regulate the differentiation of the hemangioblast to hematopoietic and endothelial cells are largely unknown. In general, much of embryonic development is coordinately regulated by temporal and spatial expression of transcription factors, such as the Homeobox (Hox) gene family. We and others isolated a divergent homeobox gene termed Hex (or Prh) that is preferentially expressed in hematopoietic and endothelial cells. Using in vitro Hex -/- embryonic stem (ES) cell differentiation, in vivo yolk sac hematopoietic progenitor assays, and chimeric mouse analysis, we found that Hex is required for differentiation of the hemangioblast to definitive embryonic hematopoietic progenitors and to a lesser extent endothelial cells. Therefore, Hex is a novel regulator of hemangioblast differentiation to hematopoietic and endothelial cells.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
O. Sripichai, C. M. Kiefer, N. V. Bhanu, T. Tanno, S.-J. Noh, S.-H. Goh, J. E. Russell, C. L. Rognerud, C.-N. Ou, P. A. Oneal, et al.
Cytokine-mediated increases in fetal hemoglobin are associated with globin gene histone modification and transcription factor reprogramming
Blood,
September 10, 2009;
114(11):
2299 - 2306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Soufi, P. Noy, M. Buckle, A. Sawasdichai, K. Gaston, and P.-S. Jayaraman
CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding
Nucleic Acids Res.,
June 1, 2009;
37(10):
3288 - 3300.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Jankovic, P. Gorello, T. Liu, S. Ehret, R. La Starza, C. Desjobert, F. Baty, M. Brutsche, P.-S. Jayaraman, A. Santoro, et al.
Leukemogenic mechanisms and targets of a NUP98/HHEX fusion in acute myeloid leukemia
Blood,
June 15, 2008;
111(12):
5672 - 5682.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Pearson, P. Sroczynska, G. Lacaud, and V. Kouskoff
The stepwise specification of embryonic stem cells to hematopoietic fate is driven by sequential exposure to Bmp4, activin A, bFGF and VEGF
Development,
April 15, 2008;
135(8):
1525 - 1535.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. R. Perlingeiro
Endoglin is required for hemangioblast and early hematopoietic development
Development,
August 15, 2007;
134(16):
3041 - 3048.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G.-M. Zou, M.-H. Luo, A. Reed, M. R. Kelley, and M. C. Yoder
Ape1 regulates hematopoietic differentiation of embryonic stem cells through its redox functional domain
Blood,
March 1, 2007;
109(5):
1917 - 1922.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kubo, V. Chen, M. Kennedy, E. Zahradka, G. Q. Daley, and G. Keller
The homeobox gene HEX regulates proliferation and differentiation of hemangioblasts and endothelial cells during ES cell differentiation
Blood,
June 15, 2005;
105(12):
4590 - 4597.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. J. Donaldson, M. Chapman, S. Kinston, J. R. Landry, K. Knezevic, S. Piltz, N. Buckley, A. R. Green, and B. Gottgens
Genome-wide identification of cis-regulatory sequences controlling blood and endothelial development
Hum. Mol. Genet.,
March 1, 2005;
14(5):
595 - 601.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Djavani, I. Topisirovic, J. C. Zapata, M. Sadowska, Y. Yang, J. Rodas, I. S. Lukashevich, C. W. Bogue, C. D. Pauza, K. L. B. Borden, et al.
The Proline-Rich Homeodomain (PRH/HEX) Protein Is Down-Regulated in Liver during Infection with Lymphocytic Choriomeningitis Virus
J. Virol.,
February 15, 2005;
79(4):
2461 - 2473.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hallaq, E. Pinter, J. Enciso, J. McGrath, C. Zeiss, M. Brueckner, J. Madri, H. C. Jacobs, C. M. Wilson, H. Vasavada, et al.
A null mutation of Hhex results in abnormal cardiac development, defective vasculogenesis and elevated Vegfa levels
Development,
October 15, 2004;
131(20):
5197 - 5209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Urbich and S. Dimmeler
Endothelial Progenitor Cells: Characterization and Role in Vascular Biology
Circ. Res.,
August 20, 2004;
95(4):
343 - 353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. E. Swingler, K. L. Bess, J. Yao, S. Stifani, and P.-S. Jayaraman
The Proline-rich Homeodomain Protein Recruits Members of the Groucho/Transducin-like Enhancer of Split Protein Family to Co-repress Transcription in Hematopoietic Cells
J. Biol. Chem.,
August 13, 2004;
279(33):
34938 - 34947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Sato, V. W. Keng, T. Yamamoto, S. Kasamatsu, T. Ban, H. Tanaka, S.-i. Satoh, K. Yamada, and T. Noguchi
Identification and Characterization of the Hematopoietic Cell-Specific Enhancer-Like Element of the Mouse Hex Gene
J. Biochem.,
February 1, 2004;
135(2):
259 - 268.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
