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Prepublished online as a Blood First Edition Paper on July 10, 2003; DOI 10.1182/blood-2003-02-0663. This Article Full Text (PDF) All Versions of this Article: 2003-02-0663v1 102/8/2728    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liles, W C Articles by Dale, D. C Search for Related Content PubMed PubMed Citation Articles by Liles, W C Articles by Dale, D. C Social Bookmarking                   What's this? Submitted March 3, 2003 Accepted June 26, 2003 Mobilization of hematopoietic progenitor cells in healthy volunteers by AMD3100, a CXCR4 antagonist W C Liles, Hal E Broxmeyer, Elin Rodger, Brent Wood, Kai Hubel, Scott Cooper, Giao Hangoc, Gary J Bridger, Geoffrey W Henson, Gary Calandra, and David C Dale* Department of Medicine, University of Washington, Seattle, WA, USA Department of Micro/Immunol & Walther Oncol. Center, Indiana University School of Medicine, Indianapolis, IN, USA AnorMED Inc., Langley, BC, Canada * Corresponding author; email: dcdale{at}u.washington.edu. Stromal cell-derived factor 1 (SDF1/CXCL12) and its cognate receptor, CXCR4, play key regulatory roles in CD34+ cell trafficking. We investigated whether AMD3100, a selective CXCR4 antagonist, could mobilize hematopoietic progenitor cells from marrow to peripheral blood in healthy human volunteers. Initially, 10 individuals received a single dose of AMD3100 (80 µg/kg subcutaneously), which induced rapid, generalized leukocytosis associated with an increase in peripheral blood CD34+ cells, representing pluripotent hematopoietic progenitors by in vitro colony-forming unit assays, from 3.8±0.5/µL to 20.7±3.5/µl at 6h. Subsequent dose-response studies showed a maximum increase in circulating CD34+ cells from 2.6±0.3/µL to 40.4±3.4/µL at 9h after 240 mg/kg of AMD3100. Serial administration of AMD3100 (80 µg/kg/d x 3d) caused consistent, reversible increases in peripheral blood CD34+ cells. AMD3100 administration was well-tolerated with only mild, transient toxicity. These findings suggest potential clinical application of AMD3100 for CD34+ cell mobilization and collection for hematopoietic stem cell transplantation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Skokowa, J. P. Fobiwe, L. Dan, B. 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