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Prepublished online as a Blood First Edition Paper on June 12, 2003; DOI 10.1182/blood-2003-03-0693. This Article Full Text (PDF) All Versions of this Article: 2003-03-0693v1 102/7/2678    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shet, A. S Articles by Hebbel, R. P Search for Related Content PubMed PubMed Citation Articles by Shet, A. S Articles by Hebbel, R. P Related Collections Related Article in Blood Online Social Bookmarking                   What's this? Submitted March 5, 2003 Accepted June 2, 2003 Sickle blood contains tissue factor positive microparticles derived from endothelial cells and monocytes Arun S Shet*, Omer Aras, Kalpna Gupta, Matthew J Hass, Douglas J Rausch, Nabil Saba, Louann Koopmeiners, Nigel S Key, and Robert P Hebbel Division of Hematology, Oncology and Transplantation, University of Minnesota Medical School, Minneapolis, MN, USA; Vascular Biology Center, University of Minnesota Medical School, Minneapolis, MN, USA Division of Hematology, Oncology and Transplantation, University of Minnesota Medical School, Minneapolis, MN, USA Section of Hematology and Oncology, Hennepin County Medical Center, Minneapolis, MN, USA; Division of Hematology, Oncology and Transplantation, University of Minnesota Medical School, Minneapolis, MN, USA Section of Hematology and Oncology, Hennepin County Medical Center, Minneapolis, MN, USA * Corresponding author; email: shetx001{at}umn.edu. Blood microparticles (MPs) in sickle cell disease (SCD) are reportedly derived only from erythrocytes and platelets. Yet in SCD, endothelial cells and monocytes are activated and abnormally express tissue factor (TF). Thus, sickle blood might contain TF positive MPs derived from these cells. Using flow cytometry to enumerate and characterize MPs, we found total MPs to be elevated in crisis (p=0.0001) and steady state (p=0.02) sickle subjects versus controls. These MPs were derived from erythrocytes, platelets, monocytes, and endothelial cells. Erythrocyte-derived MPs were elevated in sickle crisis (p=0.0001) and steady state (p=0.02) versus controls, as were monocyte-derived MPs (p=0.0004 and p=0.009, respectively). Endothelial and platelet-derived MPs were elevated in sickle crisis versus controls. Total TF positive MPs were elevated in sickle crisis versus steady state (p=0.004)and controls (p<0.0001) and were derived from both monocytes and endothelial cells. Sickle MPs shortened plasma-clotting time compared with control MPs, and a TF antibody partially inhibited this procoagulant activity. Markers of coagulation were elevated in sickle patients versus controls and correlated with total MPs and TF positive MPs (p<0.01 for both). These data support the concept that SCD is an inflammatory state with monocyte and endothelial activation and abnormal TF activity. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: ...And so on to viscosity Stephen H. Embury Blood 2003 102: 2318-2319. [Full Text] [PDF] This article has been cited by other articles: J. I. Zwicker, H. A. Liebman, D. Neuberg, R. Lacroix, K. A. Bauer, B. C. Furie, and B. Furie Tumor-Derived Tissue Factor-Bearing Microparticles Are Associated With Venous Thromboembolic Events in Malignancy Clin. Cancer Res., November 15, 2009; 15(22): 6830 - 6840. [Abstract] [Full Text] [PDF] K. I. 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