SEARCH:   Advanced

Blood, 15 February 2004, Vol. 103, No. 4, pp. 1325-1332. Prepublished online as a Blood First Edition Paper on October 16, 2003; DOI 10.1182/blood-2003-03-0799. This Article Full Text (PDF) All Versions of this Article: 2003-03-0799v1 103/4/1325    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kaufman, D. S Articles by Thomson, J. A Search for Related Content PubMed PubMed Citation Articles by Kaufman, D. S Articles by Thomson, J. A Social Bookmarking                   What's this? Submitted March 14, 2003 Accepted October 7, 2003 Functional endothelial cells derived from rhesus monkey embryonic stem cells Dan S Kaufman*, Rachel L Lewis, Eric T Hanson, Robert Auerbach, Johanna Plendl, and James A Thomson National Primate Research Center, University of Wisconsin, Madison, WI, USA; Department of Medicine/Hematology, University of Wisconsin, Madison, WI, USA Laboratory of Developmental Biology, University of Wisconsin, Madison, WI, USA Department of Anatomy, University of Wisconsin, Madison, WI, USA Fachbereich Veterinarmedizin, Institut fur Veterinar-Anatomie, Freie Universitat Berlin, Berlin, Germany * Corresponding author; email: kaufm020{at}umn.edu. We have used rhesus monkey embryonic stem (ES) cells to study endothelial cell development. Rhesus ES cells (R366.4 cell line) exposed to medium containing VEGF, bFGF, IGF, and EGF assumed a uniform endothelial cell morphology and could be propagated and expanded with a consistent phenotype and normal karyotype. When placed in Matrigel, these rhesus ES cell-derived endothelial cells (RESDECs) formed capillary-like structures characteristic of endothelial cells. Immunohistochemical and flow cytometric analysis of RESDECs showed that they take up acetylated LDL, express CD146, von Willebrand factor, and the integrin av3, and bind the lectin Ulex Europaeus Agglutinin-1. These cells also express the VEGF receptor Flk-1 and secrete VEGF. When introduced in a Matrigel plug implanted subcutaneously in mice, RESDECs formed intact vessels and recruited new endothelial cell growth. In vivo function was demonstrated by co-injection of RESDECs with murine tumor cells subcutaneously into immunocompromised adult mice. RESDECs injected alone did not form measurable tumors. Tumor cells grew more rapidly and had increased vascularization when co-injected with the RESDECs. Immunohistochemical staining demonstrated that the RESDECs participated in forming the tumor neo-vasculature. RESDECs provide a novel means to examine the mechanisms of endothelial cell development, and may open up new therapeutic strategies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. S. Woll, J. K. Morris, M. S. Painschab, R. K. Marcus, A. D. Kohn, T. L. Biechele, R. T. Moon, and D. S. Kaufman Wnt signaling promotes hematoendothelial cell development from human embryonic stem cells Blood, January 1, 2008; 111(1): 122 - 131. [Abstract] [Full Text] [PDF] Z. Li, J. C. Wu, A. Y. Sheikh, D. Kraft, F. Cao, X. Xie, M. Patel, S. S. Gambhir, R. C. Robbins, J. P. Cooke, et al. Differentiation, Survival, and Function of Embryonic Stem Cell Derived Endothelial Cells for Ischemic Heart Disease Circulation, September 11, 2007; 116(11_suppl): I-46 - I-54. [Abstract] [Full Text] [PDF] L. S. Ferreira, S. Gerecht, H. F. Shieh, N. Watson, M. A. Rupnick, S. M. Dallabrida, G. Vunjak-Novakovic, and R. Langer Vascular Progenitor Cells Isolated From Human Embryonic Stem Cells Give Rise to Endothelial and Smooth Muscle Like Cells and Form Vascular Networks In Vivo Circ. Res., August 3, 2007; 101(3): 286 - 294. [Abstract] [Full Text] [PDF] S. Levenberg, J. Zoldan, Y. Basevitch, and R. Langer Endothelial potential of human embryonic stem cells Blood, August 1, 2007; 110(3): 806 - 814. [Abstract] [Full Text] [PDF] N. L. Boyd, S. K. Dhara, R. Rekaya, E. A. Godbey, K. Hasneen, R. R. Rao, F. D. West III, B. A. Gerwe, and S. L. Stice BMP4 Promotes Formation of Primitive Vascular Networks in Human Embryonic Stem Cell-Derived Embryoid Bodies Experimental Biology and Medicine, June 1, 2007; 232(6): 833 - 843. [Abstract] [Full Text] [PDF] Q. Xiao, L. Zeng, Z. Zhang, Y. Hu, and Q. Xu Stem cell-derived Sca-1+ progenitors differentiate into smooth muscle cells, which is mediated by collagen IV-integrin {alpha}1/beta1/{alpha}v and PDGF receptor pathways Am J Physiol Cell Physiol, January 1, 2007; 292(1): C342 - C352. [Abstract] [Full Text] [PDF] Q. Xiao, L. Zeng, Z. Zhang, A. Margariti, Z. A Ali, K. M. Channon, Q. Xu, and Y. Hu Sca-1+ Progenitors Derived From Embryonic Stem Cells Differentiate Into Endothelial Cells Capable of Vascular Repair After Arterial Injury Arterioscler Thromb Vasc Biol, October 1, 2006; 26(10): 2244 - 2251. [Abstract] [Full Text] [PDF] L. Zeng, Q. Xiao, A. Margariti, Z. Zhang, A. Zampetaki, S. Patel, M. C. Capogrossi, Y. Hu, and Q. Xu HDAC3 is crucial in shear- and VEGF-induced stem cell differentiation toward endothelial cells J. Cell Biol., September 25, 2006; 174(7): 1059 - 1069. [Abstract] [Full Text] [PDF] H. Zhang, K. Saeki, A. Kimura, K. Saeki, M. Nakahara, M. Doshi, Y. Kondo, T. Nakano, and A. Yuo Efficient and Repetitive Production of Hematopoietic and Endothelial Cells from Feeder-Free Monolayer Culture System of Primate Embryonic Stem Cells Biol Reprod, February 1, 2006; 74(2): 295 - 306. [Abstract] [Full Text] [PDF] J. C. Brodie and H. D. Humes Stem Cell Approaches for the Treatment of Renal Failure Pharmacol. Rev., September 1, 2005; 57(3): 299 - 313. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020