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Prepublished online as a Blood First Edition Paper on August 28, 2003; DOI 10.1182/blood-2003-03-0824. This Article Full Text (PDF) All Versions of this Article: 2003-03-0824v1 103/2/630    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kondo, E. Articles by Takahashi, T. Search for Related Content PubMed PubMed Citation Articles by Kondo, E. Articles by Takahashi, T. Social Bookmarking                   What's this? Submitted March 19, 2003 Accepted August 26, 2003 Identification of novel CTL epitopes of CMV-pp65 presented by a variety of HLA alleles Eisei Kondo, Yoshiki Akatsuka*, Kiyotaka Kuzushima, Kunio Tsujimura, Shoji Asakura, Kohei Tajima, Yoshitoyo Kagami, Yoshihisa Kodera, Mitsune Tanimoto, Yasuo Morishima, and Toshitada Takahashi Division of Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan Department of Hematology and Chemotherapy, Aichi Cancer Center Hospital, Nagoya, Japan Department of Hematology, Japanese Red Cross Nagoya First Hospital, Nagoya, Japan Department of Internal Medicine II, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan * Corresponding author; email: yakatsuk{at}aichi-cc.jp. Cytomegalovirus (CMV) specific T-cell immunity plays an important role in protection from CMV disease in immunocompromised patients. Identification of cytotoxic T-lymphocyte (CTL) epitopes is essential for monitoring T-cell immunity and also for immunotherapy. In this and previous studies, CMV pp65-specific CTL lines were successfully generated from all of 11 CMV-seropositive healthy donors, using pp65-transduced CD40-activated B (CD40-B) cells as antigen presenting cells. By use of enzyme-linked immunospot (ELISPOT) assays, individual CTL epitopes could be mapped with truncated forms of the pp65 gene. For HLA alleles with a known binding motif, CTL epitopes within the defined regions were predicted by computer algorithm. For HLA alleles without a known binding motif (HLA-Cw*0801, -Cw*1202 and -Cw*1502), the epitopes were alternatively identified by step-by-step truncations of the pp65 gene. Through this study, a total of 14 novel CTL epitopes of CMV-pp65 were identified. Interestingly, three peptides were found to be presented by two different HLA class I alleles or subtypes. Moreover, use of CD40-B cells pulsed with a mixture of synthetic peptides led to generation of pp65-specific CTL lines from a part of seronegative donors. The study thus demonstrated an efficient strategy for identifying CTL epitopes presented by a variety of HLA alleles. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Schub, I. G. Schuster, W. Hammerschmidt, and A. Moosmann CMV-Specific TCR-Transgenic T Cells for Immunotherapy J. Immunol., November 15, 2009; 183(10): 6819 - 6830. [Abstract] [Full Text] [PDF] A. N. Hasan, W. J. Kollen, D. Trivedi, A. Selvakumar, B. Dupont, M. Sadelain, and R. J. O'Reilly A Panel of Artificial APCs Expressing Prevalent HLA Alleles Permits Generation of Cytotoxic T Cells Specific for Both Dominant and Subdominant Viral Epitopes for Adoptive Therapy J. Immunol., August 15, 2009; 183(4): 2837 - 2850. [Abstract] [Full Text] [PDF] P. Jagannathan, C. M. Osborne, C. 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