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Prepublished online as a Blood First Edition Paper on July 31, 2003; DOI 10.1182/blood-2003-03-0919. This Article Full Text (PDF) All Versions of this Article: 2003-03-0919v1 102/10/3569    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Klippel, S. Articles by Pahl, H. L Search for Related Content PubMed PubMed Citation Articles by Klippel, S. Articles by Pahl, H. L Related Collections Related Letter in Blood Online Social Bookmarking                   What's this? Submitted March 25, 2003 Accepted July 21, 2003 Quantification of PRV-1 mRNA distinguishes Polycythemia vera from secondary erythrocytosis Steffen Klippel, Elisabeth Strunck, Snezana Temerinac, Anthony J Bench, Gerold Meinhardt, Ursula Mohr, Rosi Leichtle, Anthony R Green, Martin Griesshammer, Hermann Heimpel, and Heike L Pahl* Department of Experimental Anaesthesiology, University Hospital Freiburg, Freiburg, Germany Department of Hematology/Oncology, Klinikum Innenstadt, Munich, Germany Department of Hematology/Oncology, University Hospital Freiburg, Freiburg, Germany Department of Hematology and Oncology, University Hospital Ulm, Ulm, Germany Department of Haematology, University of Cambridge, Cambridge, United Kingdom * Corresponding author; email: pahl{at}uni-freiburg.de. To date, the diagnosis of Polycythemia vera (PV) relies on clinical criteria. We have recently described the overexpression of a hematopoietic receptor, PRV-1 (polycythemia rubra vera-1), in patients with PV. Here, we report a quantitative RT-PCR assay for the measurement of PRV-1 mRNA levels. We have determined PRV-1 expression in 75 patients with PV, 11 patients with secondary erythrocytosis (SE) as well as in 80 healthy controls. PV patients express significantly higher amounts of PRV-1 than healthy controls or patients with SE (p<0.00001). Because there is no overlap between the PRV-1 expression in PV patients versus healthy controls or SE patients, the assay has a very high sensitivity and specificity for the diagnosis of PV in our population. In patients with erythrocytosis, the quantitative RT-PCR assay described here therefore provides a rapid, highly specific and sensitive tool for the diagnosis of PV. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? 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