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Prepublished online as a Blood First Edition Paper on July 17, 2003; DOI 10.1182/blood-2003-03-0966. This Article Full Text (PDF) All Versions of this Article: 2003-03-0966v1 102/9/3238    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ward, A. C Articles by Lieschke, G. J Search for Related Content PubMed PubMed Citation Articles by Ward, A. C Articles by Lieschke, G. J Social Bookmarking                   What's this? Submitted March 28, 2003 Accepted June 23, 2003 The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish Alister C Ward, Dora O McPhee, Melanie M Condron, Sony Varma, Stephen H Cody, Sara M Onnebo, Barry H Paw, Leonard I Zon, and Graham J Lieschke* Cytokine Biology Laboratory, Ludwig Institute for Cancer Research, Parkville, VIC, Australia Centre for Cellular and Molecular Biology, Deakin University, Burwood, VIC, Australia Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA * Corresponding author; email: Graham.Lieschke{at}ludwig.edu.au. The spi1 (pu.1) gene has recently been identified as a useful marker of early myeloid cells in zebrafish. In order to enhance the versatility of this organism as a model for studying myeloid development, the promoter of this gene has been isolated and characterized. Transient transgenesis revealed that a 5.3 kb promoter fragment immediately upstream of the spi1 coding sequence was sufficient to drive expression of EGFP in injected embryos in a manner that largely recapitulated the native spi1 gene expression pattern. This fragment was successfully used to produce a germline transgenic line of zebrafish with EGFP-expressing myeloid cells. These TG(spi1:EGFP)pA301 transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Lin, J. A. Loughman, B. H. Zinselmeyer, M. J. Miller, and M. G. Caparon Streptolysin S Inhibits Neutrophil Recruitment during the Early Stages of Streptococcus pyogenes Infection Infect. Immun., November 1, 2009; 77(11): 5190 - 5201. [Abstract] [Full Text] [PDF] O. W. Stockhammer, A. Zakrzewska, Z. Hegedus, H. P. Spaink, and A. H. Meijer Transcriptome Profiling and Functional Analyses of the Zebrafish Embryonic Innate Immune Response to Salmonella Infection J. Immunol., May 1, 2009; 182(9): 5641 - 5653. [Abstract] [Full Text] [PDF] C. Liongue, C. J. Hall, B. A. O'Connell, P. Crosier, and A. C. Ward Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration Blood, March 12, 2009; 113(11): 2535 - 2546. [Abstract] [Full Text] [PDF] R. M. B. Costa, X. Soto, Y. Chen, A. M. Zorn, and E. Amaya spib is required for primitive myeloid development in Xenopus Blood, September 15, 2008; 112(6): 2287 - 2296. [Abstract] [Full Text] [PDF] D. Carradice and G. J. Lieschke Zebrafish in hematology: sushi or science? Blood, April 1, 2008; 111(7): 3331 - 3342. [Abstract] [Full Text] [PDF] G. J. Lieschke Fluorescent neutrophils throw the spotlight on inflammation Blood, December 15, 2006; 108(13): 3961 - 3962. [Full Text] [PDF] S. A. Renshaw, C. A. Loynes, D. M.I. Trushell, S. Elworthy, P. W. Ingham, and M. K.B. Whyte A transgenic zebrafish model of neutrophilic inflammation Blood, December 15, 2006; 108(13): 3976 - 3978. [Abstract] [Full Text] [PDF] H.-F. Lin, D. Traver, H. Zhu, K. Dooley, B. H. Paw, L. I. Zon, and R. I. Handin Analysis of thrombocyte development in CD41-GFP transgenic zebrafish Blood, December 1, 2005; 106(12): 3803 - 3810. [Abstract] [Full Text] [PDF] J. T. Shin, J. R. Priest, I. Ovcharenko, A. Ronco, R. K. Moore, C. G. Burns, and C. A. MacRae Human-zebrafish non-coding conserved elements act in vivo to regulate transcription Nucleic Acids Res., September 22, 2005; 33(17): 5437 - 5445. [Abstract] [Full Text] [PDF] K. Hsu, D. Traver, J. L. Kutok, A. Hagen, T.-X. Liu, B. H. Paw, J. Rhodes, J. N. Berman, L. I. Zon, J. P. Kanki, et al. The pu.1 promoter drives myeloid gene expression in zebrafish Blood, September 1, 2004; 104(5): 1291 - 1297. [Abstract] [Full Text] [PDF] D. M. Langenau, A. A. Ferrando, D. Traver, J. L. Kutok, J.-P. D. Hezel, J. P. Kanki, L. I. Zon, A. T. Look, and N. S. Trede In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish PNAS, May 11, 2004; 101(19): 7369 - 7374. [Abstract] [Full Text] [PDF]

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