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Prepublished online as a Blood First Edition Paper on September 25, 2003; DOI 10.1182/blood-2003-04-1069. This Article Full Text (PDF) All Versions of this Article: 2003-04-1069v1 103/3/1099    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wagner, C. Articles by Hansch, G. M. Search for Related Content PubMed PubMed Citation Articles by Wagner, C. Articles by Hansch, G. M. Related Collections Related Letters in Blood Online Social Bookmarking                   What's this? Submitted April 7, 2003 Accepted September 21, 2003 Granzyme B and perforin: constitutive expression in human polymorphonuclear neutrophils (PMN) Christof Wagner, Christof Iking-Konert, Birgit Denefleh, Sabine Stegmaier, Friederike Hug, and Gertrud Maria Hansch* Institute of Immunology, University of Heidelberg, Heidelberg, Germany * Corresponding author; email: n50{at}ix.urz.uni-heidelberg.de. Polymorphonuclear neutrophils (PMN) produce an abundance of bactericidal and cytotoxic molecules consistent with their role as first line defence against bacterial infection. PMN, however, also cause efficient cellular cytotoxicity when targeted through Fc-receptors to appropriate antibody-coated target cells. Though this so-called antibody-dependent cellular cytotoxicity (ADCC) has been described many years ago, the mechanism of killing is still elusive. We now found that PMN contain perforin and granzyme B, the two molecules known as the cytotoxic entity of NK-cells and of cytotoxic T-lymphocytes as well. Lysates of PMN were lytic for chicken erythrocytes in a time-, temperature-, and Ca 2+-dependent manner. Moreover, apoptosis of Jurkat cells was induced, consistent with the observation that the PMN-lysates contain enzymatically active granzyme B. Taken together, our data provide evidence for the presence of perforin and granzyme B within the cytotoxic arsenal of PMN. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letters in Blood Online: Granzyme A: an additional weapon of human polymorphonuclear neutrophils (PMNs) in innate immunity? Kathrin Hochegger, Philipp Eller, and Alexander R. Rosenkranz Blood 2004 103: 1176. [Full Text] [PDF] Human neutrophils lack granzyme A, granzyme B, and perforin Sunil S. Metkar and Christopher J. Froelich Blood 2004 104: 905-906. 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