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Prepublished online as a Blood First Edition Paper on July 10, 2003; DOI 10.1182/blood-2003-04-1094. This Article Full Text (PDF) All Versions of this Article: 2003-04-1094v1 102/9/3196    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schmerer, M. Articles by Evans, T. Search for Related Content PubMed PubMed Citation Articles by Schmerer, M. Articles by Evans, T. Social Bookmarking                   What's this? Submitted April 8, 2003 Accepted July 3, 2003 Primitive erythropoiesis is regulated by Smad-dependent signaling in post-gastrulation mesoderm Matthew Schmerer and Todd Evans* Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY, USA * Corresponding author; email: tevans{at}aecom.yu.edu. The Bone Morphogenetic Proteins (BMPs) are required for the development of ventral mesoderm, which contributes to the ventral blood island and primitive (yolk sac stage) hematopoiesis. Primitive erythropoiesis is defective when BMP signaling is blocked during gastrulation of Xenopus embryos. This phenotype might be attributed to changes in mesoderm patterning leading indirectly to altered erythropoiesis. We developed an inducible system in order to block BMP signaling in a controlled fashion at later time points in development. For this purpose, an inhibitory Smad, xSmad6, was fused to the estrogen receptor ligand binding domain. We show that ER-xSmad6 is inactive when expressed in developing embryos, but its activity is induced by estradiol. When induced early in development, ER-xSmad6 causes a dorsalized phenotype, equivalent to over-expression of native xSmad6. When ER-xSmad6 is induced after gastrulation, there is a specific defect in primitive erythropoiesis without any apparent effect on axial patterning. Our results identify an embryonic signal that is Smad-dependent, is required for maintaining expression of GATA-1, and functions within mesoderm and not the overlying ectoderm. Thus, BMP signaling is necessary both during mesoderm patterning and also following early specification events for proper regulation of the primitive erythroid lineage. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: U. Blank, G. Karlsson, and S. Karlsson Signaling pathways governing stem-cell fate Blood, January 15, 2008; 111(2): 492 - 503. [Abstract] [Full Text] [PDF] C. Durand, C. Robin, K. Bollerot, M. H. Baron, K. Ottersbach, and E. Dzierzak Embryonic stromal clones reveal developmental regulators of definitive hematopoietic stem cells PNAS, December 26, 2007; 104(52): 20838 - 20843. [Abstract] [Full Text] [PDF] L. J. McReynolds, S. Gupta, M. E. Figueroa, M. C. Mullins, and T. Evans Smad1 and Smad5 differentially regulate embryonic hematopoiesis Blood, December 1, 2007; 110(12): 3881 - 3890. [Abstract] [Full Text] [PDF] O. Tanabe, Y. Shen, Q. Liu, A. D. Campbell, T. Kuroha, M. Yamamoto, and J. D. Engel The TR2 and TR4 orphan nuclear receptors repress Gata1 transcription Genes & Dev., November 1, 2007; 21(21): 2832 - 2844. [Abstract] [Full Text] [PDF] B. T. Zafonte, S. Liu, M. Lynch-Kattman, I. Torregroza, L. Benvenuto, M. Kennedy, G. Keller, and T. Evans Smad1 expands the hemangioblast population within a limited developmental window Blood, January 15, 2007; 109(2): 516 - 523. [Abstract] [Full Text] [PDF] F. Nakazawa, H. Nagai, M. Shin, and G. Sheng Negative regulation of primitive hematopoiesis by the FGF signaling pathway Blood, November 15, 2006; 108(10): 3335 - 3343. [Abstract] [Full Text] [PDF] M. Schmerer, I. Torregroza, A. Pascal, M. Umbhauer, and T. Evans STAT5 acts as a repressor to regulate early embryonic erythropoiesis Blood, November 1, 2006; 108(9): 2989 - 2997. [Abstract] [Full Text] [PDF] S. Gupta, H. Zhu, L. I. Zon, and T. Evans BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis Development, June 1, 2006; 133(11): 2177 - 2187. [Abstract] [Full Text] [PDF] U. J. Pyati, A. E. Webb, and D. Kimelman Transgenic zebrafish reveal stage-specific roles for Bmp signaling in ventral and posterior mesoderm development Development, May 15, 2005; 132(10): 2333 - 2343. [Abstract] [Full Text] [PDF] S. Martinovic, S. Mazic, V. Kisic, N. Basic, J. Jakic-Razumovic, F. Borovecki, D. Batinic, P. Simic, L. Grgurevic, B. Labar, et al. Expression of Bone Morphogenetic Proteins in Stromal Cells from Human Bone Marrow Long-term Culture J. Histochem. Cytochem., September 1, 2004; 52(9): 1159 - 1167. [Abstract] [Full Text] [PDF]

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