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Blood, 1 May 2004, Vol. 103, No. 9, pp. 3313-3319. Prepublished online as a Blood First Edition Paper on January 8, 2004; DOI 10.1182/blood-2003-04-1121. This Article Full Text (PDF) All Versions of this Article: 2003-04-1121v1 103/9/3313    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by BENSIDHOUM, M. Articles by LOPEZ, M. Search for Related Content PubMed PubMed Citation Articles by BENSIDHOUM, M. Articles by LOPEZ, M. Social Bookmarking                   What's this? Submitted April 18, 2003 Accepted December 6, 2003 Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the nod/scid mouse. Their role in supporting human CD34 cell engraftment Morad BENSIDHOUM, Alain CHAPEL, Sabine FRANCOIS, Christelle DEMARQUAY, Christelle MAZURIER, Loic FOUILLARD, Sandrine BOUCHET, Jean Marc BERTHO, Patrick GOURMELON, Jocelyne AIGUEPERSE, Pierre CHARBORD, Norbert Claude GORIN, Dominique THIERRY, and Manuel LOPEZ* Hematologie, Laboratoire de Therapie Cellulaire et de Radioprotection Accidentelle, Faculte de Medecine Saint Antoine et IRSN, EA 1638 et INSERM U76, Paris, France; Institut de Radioprotection et de Surete Nucleaire, Fontenay Aux Roses, France Institut de Radioprotection et de Surete Nucleaire, Fontenay Aux Roses, France Hematologie, Laboratoire de Therapie Cellulaire et de Radioprotection Accidentelle, Faculte de Medecine Saint Antoine et IRSN, EA 1638 et INSERM U76, Paris, France Laboratoire d'Hematopoiese, Faculte de Medecine, Tours, France * Corresponding author; email: manuel.lopez{at}chusa.jussieu.fr. The Stro-1 antigen potentially defines a mesenchymal stem cell (MSC) progenitor subset. We here report on the role of human ex vivo expanded selected-Stro-1+ or -Stro-1- MSC subsets on the engrafment of human CD34+ cord blood cells in the NOD/SCID mouse model. The data show that cotransplantation of expanded Stro-1- cells with CD34+ cells resulted in a significant increase of human CD45, CD34, CD19 and CD11b cells detected in blood or in bone marrow (BM) and spleen as compared to the infusion of CD34+ cells alone. Infusion into mice of expanded Stro-1+ and Stro-1- cells (without CD34+ cells) showed that the numbers of Stro-1+-derived (as assessed by DNA analysis of human GLOBIN with quantitative PCR) were higher than Stro-1--derived cells in spleen, muscles, BM and kidneys, while more Stro-1--derived than Stro-1+-derived cells were found in lungs. The transduction of expanded Stro-1+ cells with an eGFP gene did not modify their cytokine release and their homing in NOD/SCID mouse tissues. The difference between the hemopoeitic support and the homing capabilities of expanded Stro-1+ and Stro-1- cells may be of importance for clinical therapeutic applications: Stro-1+ cells may rather be used for gene delivery in tissues while Stro-1- cells may rather be used to support hematopoietic engraftment. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Y. Li, S. Chen, J. Yuan, Y. Yang, J. Li, J. Ma, X. Wu, M. Freund, K. Pollok, H. Hanenberg, et al. Mesenchymal stem/progenitor cells promote the reconstitution of exogenous hematopoietic stem cells in Fancg-/- mice in vivo Blood, March 5, 2009; 113(10): 2342 - 2351. [Abstract] [Full Text] [PDF] C. Kyriakou, N. Rabin, A. Pizzey, A. Nathwani, and K. Yong Factors that influence short-term homing of human bone marrow-derived mesenchymal stem cells in a xenogeneic animal model Haematologica, October 1, 2008; 93(10): 1457 - 1465. [Abstract] [Full Text] [PDF] M Mouiseddine, S Francois, A Semont, A Sache, B Allenet, N Mathieu, J Frick, D Thierry, and A Chapel Human mesenchymal stem cells home specifically to radiation-injured tissues in a non-obese diabetes/severe combined immunodeficiency mouse model Br. J. Radiol., September 1, 2007; 80(Special_Issue_1): S49 - S55. [Abstract] [Full Text] [PDF] A. De Becker, P. Van Hummelen, M. Bakkus, I. Vande Broek, J. De Wever, M. De Waele, and I. Van Riet Migration of culture-expanded human mesenchymal stem cells through bone marrow endothelium is regulated by matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-3 Haematologica, April 1, 2007; 92(4): 440 - 449. [Abstract] [Full Text] [PDF] K. Sato, K. Ozaki, I. Oh, A. Meguro, K. Hatanaka, T. Nagai, K. Muroi, and K. Ozawa Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells Blood, January 1, 2007; 109(1): 228 - 234. [Abstract] [Full Text] [PDF] B. G. Galvez, M. Sampaolesi, S. Brunelli, D. Covarello, M. Gavina, B. Rossi, G. Costantin, Y. Torrente, and G. Cossu Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability J. Cell Biol., July 17, 2006; 174(2): 231 - 243. [Abstract] [Full Text] [PDF] J. L. Chan, K. C. Tang, A. P. Patel, L. M. Bonilla, N. Pierobon, N. M. Ponzio, and P. Rameshwar Antigen-presenting property of mesenchymal stem cells occurs during a narrow window at low levels of interferon-{gamma} Blood, June 15, 2006; 107(12): 4817 - 4824. [Abstract] [Full Text] [PDF] Y. Muguruma, T. Yahata, H. Miyatake, T. Sato, T. Uno, J. Itoh, S. Kato, M. Ito, T. Hotta, and K. Ando Reconstitution of the functional human hematopoietic microenvironment derived from human mesenchymal stem cells in the murine bone marrow compartment Blood, March 1, 2006; 107(5): 1878 - 1887. [Abstract] [Full Text] [PDF] D Thierry, J M Bertho, A Chapel, and P Gourmelon Cell therapy for the treatment of accidental radiation overexposure Br. J. Radiol., January 1, 2005; Supplement_27(1): 175 - 179. [Abstract] [Full Text] [PDF]

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