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Prepublished online as a Blood First Edition Paper on July 31, 2003; DOI 10.1182/blood-2003-04-1154. This Article Full Text (PDF) All Versions of this Article: 2003-04-1154v1 102/10/3575    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Suzuki, N. Articles by Yamamoto, M. Search for Related Content PubMed PubMed Citation Articles by Suzuki, N. Articles by Yamamoto, M. Social Bookmarking                   What's this? Submitted April 14, 2003 Accepted July 21, 2003 Identification and characterization of two types of erythroid progenitors that express GATA-1 at distinct levels Norio Suzuki, Naruyoshi Suwabe, Osamu Ohneda, Naoshi Obara, Shigehiko Imagawa, Xiaoqing Pan, Hozumi Motohashi, and Masayuki Yamamoto* Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Ibaraki, Japan; Institutes of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan; ERATO Environmental Response Project, University of Tsukuba, Tsukuba, Ibaraki, Japan Institutes of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan * Corresponding author; email: masi{at}tara.tsukuba.ac.jp. Transcription factor GATA-1 is essential for the development of the erythroid lineage. To ascertain whether strict control of GATA-1 expression level is necessary for achieving proper erythropoiesis, we established transgenic mouse lines expressing green fluorescent protein (GFP) under the control of the GATA-1 gene hematopoietic regulatory domain. We examined the GATA-1 expression level by exploiting the transgenic mice and found two GFP-positive hematopoietic progenitor fractions in the bone marrow. One is the GFPhigh fraction containing mainly CFU-E and proerythroblasts, which coexpress transferrin receptor, while the other is the GFPlow/transferrin receptor-negative fraction containing BFU-E. Since the intensity of green fluorescence correlates well with the expression level of GATA-1, these results indicate that GATA-1 is highly expressed in CFU-E, but low in BFU-E, suggesting that the incremental expression of GATA-1 is required for the formation of erythroid progenitors. We also examined GFP-positive fractions in the transgenic mouse spleen and fetal liver, and identified fractions containing BFU-E and CFU-E, respectively. This study also presents an efficient method for enriching the CFU-E and BFU-E from mouse hematopoietic tissues. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. Ohneda, S. Ohmori, Y. Ishijima, M. Nakano, and M. Yamamoto Characterization of a Functional ZBP-89 Binding Site That Mediates Gata1 Gene Expression during Hematopoietic Development J. Biol. Chem., October 30, 2009; 284(44): 30187 - 30199. [Abstract] [Full Text] [PDF] D. Nozawa, N. Suzuki, M. Kobayashi-Osaki, X. Pan, J. D. Engel, and M. Yamamoto GATA2-dependent and region-specific regulation of Gata2 transcription in the mouse midbrain Genes Cells, May 1, 2009; 14(5): 569 - 582. [Abstract] [Full Text] [PDF] M. Suzuki, T. Moriguchi, K. Ohneda, and M. 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