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Prepublished online as a Blood First Edition Paper on May 22, 2003; DOI 10.1182/blood-2003-04-1175. This Article Full Text (PDF) All Versions of this Article: 2003-04-1175v1 102/6/1985    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Baltus, T. Articles by Weber, C. Search for Related Content PubMed PubMed Citation Articles by Baltus, T. Articles by Weber, C. Social Bookmarking                   What's this? Submitted April 15, 2003 Accepted May 12, 2003 Oligomerization of RANTES is required for CCR1-mediated arrest but not CCR5-mediated transmigration of leukocytes on inflamed endothelium Thomas Baltus, Kim S C Weber, Zoe Johnson, Amanda E I Proudfoot, and Christian Weber* Department of Molecular Cardiovascular Research, University Hospital Aachen, Aachen, Germany Department of Anesthesiology, University Hospital Aachen, Aachen, Germany Department of Prevention of Cardiovascular Disease, Ludwig-Maximilians-University, Munich, Germany Department of Protein Biochemistry, Serono Pharmaceutical Research Institute, Geneva, Switzerland * Corresponding author; email: cweber{at}ukaachen.de. Chemokines control inflammatory leukocyte recruitment. The propensity of chemokines such as CCL5/RANTES to bind to glycosaminoglycans and to form higher order oligomers has been shown to be essential for its in vivo activity. However, the specific functional relevance of RANTES oligomerization for distinct steps of leukocyte recruitment on inflamed endothelium mediated by the RANTES receptors CCR1 and CCR5 remains undefined. We studied RANTES mutants with deficient oligomerization in an assay where recruitment of monocytes and CD45RO+CD4+ T cells is triggered by RANTES immobilized on activated endothelium under flow conditions. Notably, the formation of higher order RANTES oligomers was crucial for CCR1-mediated arrest but not for CCR5-mediated spreading/transmigration in flow or transendothelial chemotaxis of leukocytes. Efficient leukocyte arrest in flow but not transmigration may thus require the presentation of RANTES oligomers to bridge surface-bound RANTES and CCR1. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Shi, I. A. Awad, N. Jafari, S. Lin, P. Du, Z. A. Hage, R. Shenkar, C. C. Getch, M. Bredel, H. H. 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