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Prepublished online as a Blood First Edition Paper on September 22, 2003; DOI 10.1182/blood-2003-04-1216. This Article Full Text (PDF) All Versions of this Article: 2003-04-1216v1 103/3/1030    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by De Wit, D. Articles by Willems, F. Search for Related Content PubMed PubMed Citation Articles by De Wit, D. Articles by Willems, F. Social Bookmarking                   What's this? Submitted April 17, 2003 Accepted September 8, 2003 Blood plasmacytoid dendritic cell responses to CpG oligodeoxynucleotides are impaired in human newborns Dominique De Wit, Veronique Olislagers, Stanislas Goriely, Francoise Vermeulen, Hermann Wagner, Michel Goldman*, and Fabienne Willems Laboratoire de l'Immunologie Experimentale, University Libre de Bruxelles, Brussels, Belgium Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Munich, Germany * Corresponding author; email: mgoldman{at}ulb.ac.be. Plasmacytoid dendritic cells (pDC) respond to unmethylated CpG motifs present in bacterial DNA or unmethylated synthetic oligodeoxynucleotides (CpG). In order to assess the function of pDC in human newborns, IFN- production induced by CpG 2216 and phenotypic maturation of pDC in response to CpG 2006 were compared in cord blood and adult blood. We first observed that neonatal pDC displayed decreased upregulation of CD80, CD83, CD86 and CD40 whereas HLA-DR and CD54 upregulation did not differ significantly between adults and neonates. We then found that the production of IFN- in response to CpG was dramatically impaired in cord blood. This neonatal defect was detected both at protein and mRNA levels and was still present in blood of 4 day-old babies. These findings might be relevant to the increased susceptibility of human newborns to infections as well as to the use of CpG oligodeoxynucleotides as vaccine adjuvants in the neonatal period. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. R. Kollmann, J. Crabtree, A. Rein-Weston, D. Blimkie, F. Thommai, X. Y. Wang, P. M. Lavoie, J. Furlong, E. S. Fortuno III, A. M. Hajjar, et al. Neonatal Innate TLR-Mediated Responses Are Distinct from Those of Adults J. 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