SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on September 22, 2003; DOI 10.1182/blood-2003-04-1304. This Article Full Text (PDF) All Versions of this Article: 2003-04-1304v1 103/1/317    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Brownstein, C. Articles by Hajjar, K. A Search for Related Content PubMed PubMed Citation Articles by Brownstein, C. Articles by Hajjar, K. A Social Bookmarking                   What's this? Submitted April 28, 2003 Accepted September 8, 2003 Annexin II mediates plasminogen-dependent matrix invasion by human monocytes: enhanced expression by macrophages Carrie Brownstein, Arunkumar B Deora, Andrew T Jacovina, Rebecca Weintraub, Menard Gertler, K M Faisal Khan, Domenick J Falcone, and Katherine A Hajjar* Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY, USA; Department of Pediatrics, Weill Medical College of Cornell University, New York, NY, USA Department of Medicine, Weill Medical College of Cornell University, New York, NY, USA Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY, USA * Corresponding author; email: khajjar{at}med.cornell.edu. Monocytes and macrophages participate in a wide variety host defense mechanisms. Annexin II, a fibrinolytic receptor, binds plasminogen and tissue plasminogen activator (t-PA) independently at the cell surface, thereby enhancing the catalytic efficiency of plasmin production. We demonstrated previously that annexin II on the surface of both cultured monocytoid cells and monocyte-derived macrophages promotes their ability to remodel extracellular matrix. Here, we demonstrate that human peripheral blood monocytes represent the major circulating annexin II-expressing cell. Annexin II supported t-PA-dependent generation of cell surface plasmin and the matrix-penetrating activity of human monocytes. Compared to polymorphonuclear leukocytes, monocytes supported a 12.9-fold greater rate of plasmin generation in the presence of exogenous t-PA, and this activity was largely attributable to annexin II. Likewise, anti-annexin II IgG directed against the t-PA-binding tail domain inhibited plasminogen-dependent, cytokine-directed monocyte migration through extracellular matrix. Upon differentiation of monocytes to macrophages, there was a 2.4-fold increase in annexin II-specific mRNA, and a 7.9-fold increase in surface annexin II. Thioglycollate-elicited peritoneal macrophages, furthermore, displayed an additional 3.8-fold increase in annexin II surface expression compared with resident cells. Thus, annexin II-mediated assembly of plasminogen and t-PA on monocyte/macrophages contributes to plasmin generation, matrix remodeling, and directed migration. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Das, T. Burke, D. R. Van Wagoner, and E. F. Plow L-Type Calcium Channel Blockers Exert an Antiinflammatory Effect by Suppressing Expression of Plasminogen Receptors on Macrophages Circ. Res., July 17, 2009; 105(2): 167 - 175. [Abstract] [Full Text] [PDF] M. Wygrecka, L. M. Marsh, R. E. Morty, I. Henneke, A. Guenther, J. Lohmeyer, P. Markart, and K. T. Preissner Enolase-1 promotes plasminogen-mediated recruitment of monocytes to the acutely inflamed lung Blood, May 28, 2009; 113(22): 5588 - 5598. [Abstract] [Full Text] [PDF] E Cockrell, R. Espinola, and K. McCrae Annexin A2: biology and relevance to the antiphospholipid syndrome Lupus, October 1, 2008; 17(10): 944 - 952. [Abstract] [PDF] Y. Zhang, Z.-H. Zhou, T. H. Bugge, and L. M. Wahl Urokinase-Type Plasminogen Activator Stimulation of Monocyte Matrix Metalloproteinase-1 Production Is Mediated by Plasmin-Dependent Signaling through Annexin A2 and Inhibited by Inactive Plasmin J. Immunol., September 1, 2007; 179(5): 3297 - 3304. [Abstract] [Full Text] [PDF] S. M. Wahl, T. Greenwell-Wild, and N. Vazquez HIV accomplices and adversaries in macrophage infection J. Leukoc. Biol., November 1, 2006; 80(5): 973 - 983. [Abstract] [Full Text] [PDF] J. A. Fulcher, S. T. Hashimi, E. L. Levroney, M. Pang, K. B. Gurney, L. G. Baum, and B. Lee Galectin-1-Matured Human Monocyte-Derived Dendritic Cells Have Enhanced Migration through Extracellular Matrix J. Immunol., July 1, 2006; 177(1): 216 - 226. [Abstract] [Full Text] [PDF] G. Cesarman-Maus, N. P. Rios-Luna, A. B. Deora, B. Huang, R. Villa, M. d. C. Cravioto, D. Alarcon-Segovia, J. Sanchez-Guerrero, and K. A. Hajjar Autoantibodies against the fibrinolytic receptor, annexin 2, in antiphospholipid syndrome Blood, June 1, 2006; 107(11): 4375 - 4382. [Abstract] [Full Text] [PDF] Y. Laumonnier, T. Syrovets, L. Burysek, and T. Simmet Identification of the annexin A2 heterotetramer as a receptor for the plasmin-induced signaling in human peripheral monocytes Blood, April 15, 2006; 107(8): 3342 - 3349. [Abstract] [Full Text] [PDF] K. Cederqvist, V. Siren, J. Petaja, A. Vaheri, C. Haglund, and S. Andersson High Concentrations of Plasminogen Activator Inhibitor-1 in Lungs of Preterm Infants With Respiratory Distress Syndrome Pediatrics, April 1, 2006; 117(4): 1226 - 1234. [Abstract] [Full Text] [PDF] E. V. Ryzhova, R. M. Vos, A. V. Albright, A. V. Harrist, T. Harvey, and F. Gonzalez-Scarano Annexin 2: a Novel Human Immunodeficiency Virus Type 1 Gag Binding Protein Involved in Replication in Monocyte-Derived Macrophages J. Virol., March 15, 2006; 80(6): 2694 - 2704. [Abstract] [Full Text] [PDF] C. C. Taggart, S.-A. Cryan, S. Weldon, A. Gibbons, C. M. Greene, E. Kelly, T. B. Low, S. J. O'Neill, and N. G. McElvaney Secretory leucoprotease inhibitor binds to NF-{kappa}B binding sites in monocytes and inhibits p65 binding J. Exp. Med., December 19, 2005; 202(12): 1659 - 1668. [Abstract] [Full Text] [PDF] D. Gveric, B. M. Herrera, and M. L. Cuzner tPA Receptors and the Fibrinolytic Response in Multiple Sclerosis Lesions Am. J. Pathol., April 1, 2005; 166(4): 1143 - 1151. [Abstract] [Full Text] [PDF] G. Ma, T. Greenwell-Wild, K. Lei, W. Jin, J. Swisher, N. Hardegen, C. T. Wild, and S. M. Wahl Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection J. Exp. Med., November 15, 2004; 200(10): 1337 - 1346. [Abstract] [Full Text] [PDF] A. B. Deora, G. Kreitzer, A. T. Jacovina, and K. A. Hajjar An Annexin 2 Phosphorylation Switch Mediates p11-dependent Translocation of Annexin 2 to the Cell Surface J. Biol. Chem., October 15, 2004; 279(42): 43411 - 43418. [Abstract] [Full Text] [PDF] C. L. Galindo, A. A. Fadl, J. Sha, and A. K. Chopra Microarray Analysis of Aeromonas hydrophila Cytotoxic Enterotoxin-Treated Murine Primary Macrophages Infect. Immun., September 1, 2004; 72(9): 5439 - 5445. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020