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Blood, 15 April 2005, Vol. 105, No. 8, pp. 3141-3148. Prepublished online as a Blood First Edition Paper on July 27, 2004August 3, 2004; DOI 10.1182/blood-2003-04-1319. This Article Full Text (PDF) All Versions of this Article: 2003-04-1319v1 2003-04-1319v2 105/8/3141    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Oda, A. Articles by Takenawa, T. Search for Related Content PubMed PubMed Citation Articles by Oda, A. Articles by Takenawa, T. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 28, 2003 Accepted June 28, 2004 WAVE/Scars in Platelets Atsushi Oda*, Hiroaki Miki, Ikuo Wada, Hideki Yamaguchi, Daisuke Yamazaki, Shiro Suetsugu, Mineba Nakajima, Akira Nakayama, Katsuya Okawa, Hiroshi Miyazaki, Kazuhiko Matsuno, Hans D Ochs, Laura M Machesky, Hiroyoshi Fujita, and Tadaomi Takenawa Laboratory of Environmental Biology, Department of Preventive Medicine, Hokkaido University School of Medicine, Sapporo, Hokkaido, Japan Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan; JST, PRESTO, Japan Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan Horizontal Medical Research Organization, Kyoto University Faculty of Medicine, Kyoto, Japan Pharmaceutical Division, Pharmaceutical Research Laboratories, Kirin Brewery Co., ltd., Takasaki, Gunma, Japan College of Medical Technology and School of Medicine, Hokkaido University, Sapporo, Japan Division of Immunology, Infectious Diseases and Rheumatology, Department of Pediatrics, University of Washington School of Medicine, Seattle, WA, USA School of Biosciences, Div. of Mol. Cell Biol., University of Birmingham, Birmingham, United Kingdom * Corresponding author; email: aoda{at}med.hokudai.ac.jp. Using specific antibodies against isoforms of WAVE (WASP family Verprolin-homologous protein, also called Scar), we demonstrated that human platelets express all three isoforms. Using an in vitro pull-down technique, the src homology 3 (SH3) domain of IRS-p53 precipitated WAVE2 from platelet lysates more efficiently than Profilin I did. The opposite was true for WAVE1, and neither precipitated WAVE3, suggesting that WAVE isoforms have different affinities to these ligands, while the SH3 domain of abl binds to all three isoforms. The three WAVE isoforms were distributed in the actin-rich Triton X-100 insoluble pellets following platelet aggregation induced by thrombin-receptor activating peptide. We also found that all three WAVE isoforms are substrates for calpain in vivo and in vitro. Although portions of these three isoforms were commonly distributed in the actin and Arp2/3-rich edge of the lamellipodia in spreading platelets, only WAVE2 remained in the cell fringe following detergent extraction/fixation of the cells. Finally, by mass spectrometry, we found that the proteins, which reportedly interact with WAVE/Scars, are present in platelets. These data suggest that the three WAVE isoforms exhibit common and distinct features, and may potentially be involved in the regulation of actin cytoskeleton in platelets CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. Sossey-Alaoui, K. Bialkowska, and E. F. Plow The miR200 Family of MicroRNAs Regulates WAVE3-dependent Cancer Cell Invasion J. Biol. Chem., November 27, 2009; 284(48): 33019 - 33029. [Abstract] [Full Text] [PDF] H. Dinh, G. M. Scholz, and J. A. Hamilton Regulation of WAVE1 expression in macrophages at multiple levels J. 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