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Prepublished online as a Blood First Edition Paper on August 21, 2003; DOI 10.1182/blood-2003-05-1602. This Article Full Text (PDF) All Versions of this Article: 2003-05-1602v1 102/13/4407    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tadros, A. Articles by Brindle, N. P J Search for Related Content PubMed PubMed Citation Articles by Tadros, A. Articles by Brindle, N. P J Social Bookmarking                   What's this? Submitted May 19, 2003 Accepted August 18, 2003 ABIN-2 protects endothelial cells from death and has a role in the anti-apoptotic effect of angiopoietin-1 Amir Tadros, David P Hughes, Benjamin J Dunmore, and Nicholas P J Brindle* Department of Surgery, University of Leicester, Leicester, United Kingdom * Corresponding author; email: npjb1{at}leicester.ac.uk. A20 binding inhibitor of NFkB activation-2, ABIN-2, is a newly identified intracellular protein that interacts with the zinc finger protein A20. ABIN-2 inhibits NFkB activity and is a possible effector of A20 regulation of NFkB. Although A20 is a potent inhibitor of endothelial apoptosis, the effect of ABIN-2 on apoptosis is not known. ABIN-2 also interacts with the endothelial receptor Tie2. This receptor is essential for blood vessel formation and promotes endothelial survival. Here we examine the effects of ABIN-2 on endothelial cell apoptosis and its potential involvement in Tie2-mediated endothelial survival. ABIN-2 was found to inhibit endothelial apoptosis and rescue cells from death following growth factor-deprivation. The inhibitors of phosphatidylinositol 3-kinase, wortmannin and LY294002, suppressed ABIN-2 inhibition of endothelial cell death. Deletion of the carboxy-terminus of ABIN-2 removed its ability to inhibit apoptosis. Expression of truncated ABIN-2 prevented the Tie2 activating ligand angiopoietin-1 from inhibiting endothelial cell death. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. P.J. Brindle, P. Saharinen, and K. Alitalo Signaling and Functions of Angiopoietin-1 in Vascular Protection Circ. Res., April 28, 2006; 98(8): 1014 - 1023. [Abstract] [Full Text] [PDF] T. Murakami, H. Takagi, K. Suzuma, I. Suzuma, H. Ohashi, D. Watanabe, T. Ojima, E. Suganami, M. Kurimoto, H. Kaneto, et al. Angiopoietin-1 Attenuates H2O2-induced SEK1/JNK Phosphorylation through the Phosphatidylinositol 3-Kinase/Akt Pathway in Vascular Endothelial Cells J. Biol. Chem., September 9, 2005; 280(36): 31841 - 31849. [Abstract] [Full Text] [PDF] Y. Arao, A. Kikuchi, M. Kishida, M. Yonekura, A. Inoue, S. Yasuda, S. Wada, K. Ikeda, and F. Kayama Stability of A+U-Rich Element Binding Factor 1 (AUF1)-Binding Messenger Ribonucleic Acid Correlates with the Subcellular Relocalization of AUF1 in the Rat Uterus upon Estrogen Treatment Mol. Endocrinol., September 1, 2004; 18(9): 2255 - 2267. [Abstract] [Full Text] [PDF]

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