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Prepublished online as a Blood First Edition Paper on September 25, 2003; DOI 10.1182/blood-2003-05-1618. This Article Full Text (PDF) All Versions of this Article: 2003-05-1618v1 103/4/1270    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sergejeva, S. Articles by Lotvall, J. Search for Related Content PubMed PubMed Citation Articles by Sergejeva, S. Articles by Lotvall, J. Social Bookmarking                   What's this? Submitted May 20, 2003 Accepted September 20, 2003 Allergen exposure-induced differences in CD34+ cell phenotype; relationship to eosinophilopoietic responses in different compartments Svetlana Sergejeva*, Anna-Karin Johansson, Carina Malmhall, and Jan Lotvall Department of Respiratory Medicine and Allergology, The Lung Pharmacology Group, Goteborg, Sweden * Corresponding author; email: Svetlana.Sergejeva{at}lungall.gu.se. We hypothesized that the allergen-induced increased number of airway eosinophils results from increased recruitment of eosinophils from bone marrow (BM) and local development of CD34+ cells into eosinophils. We also assumed that the phenotype of airway eosinophils depends on whether these cells have differentiated within BM or airway. C57BL/6 mice were sensitized and subsequently exposed to OVA on 5 consecutive days. Newly produced cells were labeled with a thymidine analog. Clonogenic activity and IL-5 release from BALf CD34+ cells were evaluated using cell-culture techniques. Allergen exposure induces increase in CD135+ primitive myeloid progenitors within the CD34+ cell population, without significant changes in total number of CD34+ cells or newly produced CD34+ cells. CD34+/IL-5R+ cells in the first stage of cell differentiation were found only in BM, arguing that early commitment of CD34+ cells into the eosinophil lineage is restricted to the BM compartment. Allergen exposure induces a shift in differentiation of BM, blood, and BALf eosinophil-lineage-committed CD34+ cells towards mature eosinophils and recruitment of these cells via blood into airway. We further demonstrate in vitro that ability to multiply persists in BALf CD34+ cells but not CD34- cells, likely via autocrine IL-5 release and IL-5-induced up-regulation of IL-5R. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. H. Kang, D. H. Lee, H. Seo, J. S. Park, K. H. Nam, S. Y. Shin, C.-S. Park, and I. Y. Chung Regulation of Functional Phenotypes of Cord Blood Derived Eosinophils by {gamma}-Secretase Inhibitor Am. J. Respir. Cell Mol. Biol., November 1, 2007; 37(5): 571 - 577. [Abstract] [Full Text] [PDF]

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