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Blood, 1 April 2004, Vol. 103, No. 7, pp. 2795-2798. Prepublished online as a Blood First Edition Paper on October 9, 2003; DOI 10.1182/blood-2003-05-1632. This Article Full Text (PDF) All Versions of this Article: 2003-05-1632v1 103/7/2795    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Babbage, G. Articles by Sahota, S. S Search for Related Content PubMed PubMed Citation Articles by Babbage, G. Articles by Sahota, S. S Social Bookmarking                   What's this? Submitted May 21, 2003 Accepted October 5, 2003 Mantle cell lymphoma with t(11;14) and unmutated or mutated VH genes expresses AID and undergoes isotype switch events Gavin Babbage, Richard Garand, Nelly Robillard, Niklas Zojer, Freda K Stevenson, and Surinder S Sahota* Tenovus Laboratory, Southampton University Hospitals, Southampton, United Kingdom Laboratoire d'Hematologie, Institut de Biologie des Hopitaux, Nantes, France * Corresponding author; email: s.s.sahota{at}soton.ac.uk. Isotype switch commonly follows onset of somatic hypermutation in the germinal center (GC), with activation induced cytidine deaminase (AID) a pre-requisite. Mantle cell lymphoma (MCL) with t(11;14) includes a subset with unmutated (UM) and a minor subset with mutated (MUT) VH genes. Here, we investigated whether switch events and AID expression occur in MCL. In 4/6 UM and 4/7 MUT MCL, alternative tumor-derived C,, transcripts were identified. AID transcripts, including a splice variant were common to both subsets. AID expression correlated with switch in 8/8 cases, but in 3/5 cases occurred with switch absent. Circle transcripts (I-Cµ/I-Cµ) were identified in 5/7 evaluated cases. In 1/12 cases, 12% of tumor cells expressed IgL-restricted sIgA. On-going switch recombination events appear to be a feature of MCL, likely restricted to a minor tumor subpopulation, with occasional variant sIg expression. UM MCL implicate origins from pre-GC B-cells, and reveal switch events at ectopic sites. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: X. Wu, J. R. Darce, S. K. Chang, G. S. Nowakowski, and D. F. Jelinek Alternative splicing regulates activation-induced cytidine deaminase (AID): implications for suppression of AID mutagenic activity in normal and malignant B cells Blood, December 1, 2008; 112(12): 4675 - 4682. [Abstract] [Full Text] [PDF] P. Perez-Duran, V. G. de Yebenes, and A. R. Ramiro Oncogenic events triggered by AID, the adverse effect of antibody diversification Carcinogenesis, December 1, 2007; 28(12): 2427 - 2433. [Abstract] [Full Text] [PDF] G. R. Kolar, D. Mehta, R. Pelayo, and J. D. Capra A novel human B cell subpopulation representing the initial germinal center population to express AID Blood, March 15, 2007; 109(6): 2545 - 2552. [Abstract] [Full Text] [PDF] J. E. J. Guikema, S. Rosati, K. Akkermans, R. J. Bende, C. J. M. van Noesel, J. H. van Krieken, M. L. Hansmann, E. Schuuring, P. M. Kluin, S. S. Sahota, et al. Quantitative RT-PCR analysis of activation-induced cytidine deaminase expression in tissue samples from mantle cell lymphoma and B-cell chronic lymphocytic leukemia patients Blood, April 1, 2005; 105(7): 2997 - 2999. [Full Text] [PDF] F. Forconi, S. S. Sahota, D. Raspadori, M. Ippoliti, G. Babbage, F. Lauria, and F. K. Stevenson Hairy cell leukemia: at the crossroad of somatic mutation and isotype switch Blood, November 15, 2004; 104(10): 3312 - 3317. [Abstract] [Full Text] [PDF]

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