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Blood, 1 March 2004, Vol. 103, No. 5, pp. 1669-1675. Prepublished online as a Blood First Edition Paper on October 23, 2003; DOI 10.1182/blood-2003-05-1670. This Article Full Text (PDF) All Versions of this Article: 2003-05-1670v1 103/5/1669    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lee, O. K Articles by Chen, T.-H. Search for Related Content PubMed PubMed Citation Articles by Lee, O. K Articles by Chen, T.-H. Social Bookmarking                   What's this? Submitted June 2, 2003 Accepted October 19, 2003 Isolation of multi-potent mesenchymal stem cells from umbilical cord blood Oscar K Lee*, Tom K Kuo, Wei-Ming Chen, Kuan-Der Lee, Shie-Liang Hsieh, and Tain-Hsiung Chen Department of Orthopaedics and Traumatology, Veterans General Hospital, Taipei, Taiwan Cancer Research Division, National Health Research Institute, Taipei, Taiwan Department of Immunology, National Yang-Ming University, Taipei, Taiwan * Corresponding author; email: kslee{at}vghtpe.gov.tw. It is well accepted that umbilical cord blood has been a source for hematopoietic stem cells. However, controversy exists as to whether cord blood can serve as a source of mesenchymal stem cells, which can differentiate into cells of different connective tissue lineages such as bone, cartilage and fat, and little success has been reported in the literature about the isolation of such cells from cord blood. Here we report a novel method to obtain single cell derived, clonally expanded mesenchymal stem cells which are of multi-lineage differentiation potential by negative immuno-selection and limiting dilution. The immunophenotype of these clonally-expanded cells are consistent with that reported for bone marrow mesenchymal stem cells. Under appropriate induction conditions, these cells can differentiate into bone, cartilage, and fat. Surprisingly, these cells were also able to differentiate into neuroglial- and hepatocyte-like cells under appropriate induction conditions and, thus, these cells may be more than mesenchymal stem cells as evidenced by their ability to differentiate into cell types of all three germ layers. In conclusion, umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical wastes. It can serve as an alternative source of mesenchymal stem cells to bone marrow. 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