SEARCH:   Advanced

Blood, 1 May 2004, Vol. 103, No. 9, pp. 3326-3335. Prepublished online as a Blood First Edition Paper on January 8, 2004; DOI 10.1182/blood-2003-05-1689. This Article Full Text (PDF) All Versions of this Article: 2003-05-1689v1 103/9/3326    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ravet, E. Articles by Pflumio, F. Search for Related Content PubMed PubMed Citation Articles by Ravet, E. Articles by Pflumio, F. Social Bookmarking                   What's this? Submitted May 28, 2003 Accepted December 19, 2003 Characterization of DNA-binding dependent and independent functions of SCL/TAL1 during human erythropoiesis Emmanuel Ravet, Damien Reynaud, Monique Titeux, Brigitte Izac, Serge Fichelson, Paul-Henri Romeo, Anne Dubart-Kupperschmitt, and Francoise Pflumio* Dept of Hematology, Institut Cochin, U567 INSERM, Paris, France * Corresponding author; email: plume{at}cochin.inserm.fr. The transcription factor TAL1 has major functions during embryonic hematopoiesis and in adult erythropoiesis and megakaryocytopoiesis. These functions rely on different TAL1 structural domains that are responsible for dimerization, transactivation and DNA binding. Previous work, most often done in mouse, have shown that some TAL1 functions do not require DNA binding. To study the role of TAL1 and the relevance of TAL1 DNA binding domain in human erythropoiesis, we developed an approach that allows an efficient enforced wild type or mutant TAL1 protein expression in human hematopoietic CD34+ cells using a lentiviral vector. Differentiation capacities of the transduced cells were studied in a culture system that distinguishes early and late erythroid development. Results indicate that enforced TAL1 expression enhances LTC-IC potential and erythroid differentiation of human CD34+ cells as shown by increased globin and PBGD gene expressions and CFU-e, BFU-e and GPA+ cell productions. Enforced expression of a TAL1 protein deleted of its DNA-binding domain (named bTAL1) mimicked most TAL1 effects except for the LTC-IC enhancement, the down regulation of the CD34 surface marker and the GPA+ cell production. These results provide the first functional indications of DNA-binding dependent and independent roles of TAL1 in human erythropoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Armstrong, P. B. de la Grange, B. Gerby, M.-C. Rouyez, J. Calvo, M. Fontenay, N. Boissel, H. Dombret, A. Baruchel, J. Landman-Parker, et al. NOTCH is a key regulator of human T-cell acute leukemia initiating cell activity Blood, February 19, 2009; 113(8): 1730 - 1740. [Abstract] [Full Text] [PDF] G. Despouy, M. Joiner, E. Le Toriellec, R. Weil, and M. H. Stern The TCL1 oncoprotein inhibits activation-induced cell death by impairing PKC{theta} and ERK pathways Blood, December 15, 2007; 110(13): 4406 - 4416. [Abstract] [Full Text] [PDF] V. Deleuze, E. Chalhoub, R. El-Hajj, C. Dohet, M. Le Clech, P.-O. Couraud, P. Huber, and D. Mathieu TAL-1/SCL and Its Partners E47 and LMO2 Up-Regulate VE-Cadherin Expression in Endothelial Cells Mol. Cell. Biol., April 1, 2007; 27(7): 2687 - 2697. [Abstract] [Full Text] [PDF] P. B. de la Grange, F. Armstrong, V. Duval, M.-C. Rouyez, N. Goardon, P.-H. Romeo, and F. Pflumio Low SCL/TAL1 expression reveals its major role in adult hematopoietic myeloid progenitors and stem cells Blood, November 1, 2006; 108(9): 2998 - 3004. [Abstract] [Full Text] [PDF] C. Capron, Y. Lecluse, A. L. Kaushik, A. Foudi, C. Lacout, D. Sekkai, I. Godin, O. Albagli, I. Poullion, F. Svinartchouk, et al. The SCL relative LYL-1 is required for fetal and adult hematopoietic stem cell function and B-cell differentiation Blood, June 15, 2006; 107(12): 4678 - 4686. [Abstract] [Full Text] [PDF] H. C. Suh, J. Gooya, K. Renn, A. D. Friedman, P. F. Johnson, and J. R. Keller C/EBP{alpha} determines hematopoietic cell fate in multipotential progenitor cells by inhibiting erythroid differentiation and inducing myeloid differentiation Blood, June 1, 2006; 107(11): 4308 - 4316. [Abstract] [Full Text] [PDF] Y. Choi, K. E. Elagib, L. L. Delehanty, and A. N. Goldfarb Erythroid Inhibition by the Leukemic Fusion AML1-ETO Is Associated with Impaired Acetylation of the Major Erythroid Transcription Factor GATA-1. Cancer Res., March 15, 2006; 66(6): 2990 - 2996. [Abstract] [Full Text] [PDF] M. A. Juarez, F. Su, S. Chun, M. J. Kiel, and S. E. Lyons Distinct Roles for SCL in Erythroid Specification and Maturation in Zebrafish J. Biol. Chem., December 16, 2005; 280(50): 41636 - 41644. [Abstract] [Full Text] [PDF] D. Reynaud, E. Ravet, M. Titeux, F. Mazurier, L. Renia, A. Dubart-Kupperschmitt, P.-H. Romeo, and F. Pflumio SCL/TAL1 expression level regulates human hematopoietic stem cell self-renewal and engraftment Blood, October 1, 2005; 106(7): 2318 - 2328. [Abstract] [Full Text] [PDF] M. A. Hall, N. J. Slater, C. G. Begley, J. M. Salmon, L. J. Van Stekelenburg, M. P. McCormack, S. M. Jane, and D. J. Curtis Functional but Abnormal Adult Erythropoiesis in the Absence of the Stem Cell Leukemia Gene Mol. Cell. Biol., August 1, 2005; 25(15): 6355 - 6362. [Abstract] [Full Text] [PDF] J. Wen, S. Huang, S. D. Pack, X. Yu, S. J. Brandt, and C. T. Noguchi Tal1/SCL Binding to Pericentromeric DNA Represses Transcription J. Biol. Chem., April 1, 2005; 280(13): 12956 - 12966. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020