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Prepublished online as a Blood First Edition Paper on September 11, 2003; DOI 10.1182/blood-2003-05-1710. This Article Full Text (PDF) All Versions of this Article: 2003-05-1710v1 103/1/313    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Trivedi, P. Articles by Faggioni, A. Search for Related Content PubMed PubMed Citation Articles by Trivedi, P. Articles by Faggioni, A. Social Bookmarking                   What's this? Submitted May 28, 2003 Accepted August 27, 2003 Infection of HHV-8 positive primary effusion lymphoma cells with a recombinant Epstein-Barr virus lead to restricted EBV latency, altered phenotype, and increased tumorigenicity without affecting TCL1 expression Pankaj Trivedi*, Kumi Takazawa, Claudia Zompetta, Laura Cuomo, Elena Anastasiadou, Antonino Carbone, Stefania Uccini, Filippo Belardelli, Kenzo Takada, Luigi Frati, and Alberto Faggioni Department of Experimental Medicine and Pathology, Istituto Pasteur-Fondazione Cenci Bolognetti, University of Rome, Rome, Italy Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan Department of Clinical Pathology, San Filippo Neri Hospital, Rome, Italy Department of Pathology and Scientific Direction, Centro di Riferimento Oncologico, National Cancer Institute, Aviano, Italy Laboratory of Virology, Istituto Superiore di Sanita, Rome, Italy Neuromed, INM, Pozzilli, Italy * Corresponding author; email: pankaj.trivedi{at}uniroma1.it. To investigate the role of EBV in the pathogenesis of PEL, we infected HHV-8 positive (HHV-8pos) but EBV negative (EBVneg) PEL lines BC-3, CRO-AP/6 and CRO-AP/3 cells with the recombinant Akata EBV strain. All EBV infected clones expressed EBER-1, EBNA-1 and LMP2A. The expression of LMP1 and LMP2B was variable. None however expressed EBNA2-6. The surface markers CD30, CD74 and Syndecan-1 were downregulated in EBV convertants. EBV infected BC-3 and CRO-AP/6 cells were highly tumorigenic in SCID mice in contrast to their respective EBV negative parental cells. However, neither the parental cells nor the virus converted counterparts expressed TCL1. The results showing that PELs upon in vitro EBV infection do not sustain latency III in spite of the absence of immune pressure indicate that the choice of EBV latent gene expression program is cell dependent. The data suggest an important role of EBV in the pathogenesis of PEL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. A. Mack and B. Sugden EBV Is Necessary for Proliferation of Dually Infected Primary Effusion Lymphoma Cells Cancer Res., September 1, 2008; 68(17): 6963 - 6968. [Abstract] [Full Text] [PDF] D. Xu, T. Coleman, J. Zhang, A. Fagot, C. Kotalik, L. Zhao, P. Trivedi, C. Jones, and L. Zhang Epstein-Barr Virus Inhibits Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication in Primary Effusion Lymphomas J. Virol., June 1, 2007; 81(11): 6068 - 6078. [Abstract] [Full Text] [PDF] E. Boulanger, L. Gerard, J. Gabarre, J.-M. Molina, C. Rapp, J.-F. Abino, J. Cadranel, S. Chevret, and E. Oksenhendler Prognostic Factors and Outcome of Human Herpesvirus 8-Associated Primary Effusion Lymphoma in Patients With AIDS J. Clin. Oncol., July 1, 2005; 23(19): 4372 - 4380. [Abstract] [Full Text] [PDF] A. Farina, R. Feederle, S. Raffa, R. Gonnella, R. Santarelli, L. Frati, A. Angeloni, M. R. Torrisi, A. Faggioni, and H.-J. Delecluse BFRF1 of Epstein-Barr Virus Is Essential for Efficient Primary Viral Envelopment and Egress J. Virol., March 15, 2005; 79(6): 3703 - 3712. [Abstract] [Full Text] [PDF] W. Fan, D. Bubman, A. Chadburn, W. J. Harrington Jr., E. Cesarman, and D. M. Knowles Distinct Subsets of Primary Effusion Lymphoma Can Be Identified Based on Their Cellular Gene Expression Profile and Viral Association J. Virol., January 15, 2005; 79(2): 1244 - 1251. [Abstract] [Full Text] [PDF]

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