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 Prepublished online as a Blood First Edition Paper on September 25, 2003; DOI 10.1182/blood-2003-06-1815.

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Submitted June 5, 2003
Accepted September 16, 2003

Long-term expansion of transplantable human fetal liver hematopoietic stem cells

Pierre Rollini*, Stefan Kaiser, Eveline Faes-van't Hull, Ursula Kapp, and Serge Leyvraz

Centre Pluridisciplinaire d'Oncologie, University Hospital, Lausanne, Switzerland
Hematology/Oncology Department, University Medical Center, Freiburg, Germany

* Corresponding author; email: Pierre.Rollini{at}chuv.hospvd.ch.

Hematopoietic stem cells (HSC), with their dual ability for self-renewal and multilineage differentiation, constitute an essential component of hematopoietic transplants. Human fetal liver (FL) represents a promising alternative HSC source, and we reported simple culture conditions allowing long-term expansion of FL hematopoietic progenitors. In the present study, the NOD/SCID mouse xenotransplantation assay was used to confirm that human FL is rich in NOD/SCID-repopulating cells (SRC), and to show that these culture conditions repeatedly maintained short and long-term SRC from various FL samples for at least 28 days. Quantitative limited dilution analysis in NOD/SCID mice demonstrated for the first time that a 10 to over a 100 fold net expansion of FL SRC could be achieved after 28 days of culture. The efficiency of this culture system may lead to an increase in the use of FL as a source of HSC for transplantation in adult patients, as previously demonstrated with umbilical cord blood under different culture conditions.


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