SEARCH:   Advanced

Blood, 15 March 2004, Vol. 103, No. 6, pp. 2055-2061. Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-06-1881. This Article Full Text (PDF) All Versions of this Article: 2003-06-1881v1 103/6/2055    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shmelkov, S. V Articles by Rafii, S. Search for Related Content PubMed PubMed Citation Articles by Shmelkov, S. V Articles by Rafii, S. Social Bookmarking                   What's this? Submitted June 11, 2003 Accepted October 13, 2003 Alternative promoters regulate transcription of the gene encoding stem cell surface protein AC133 Sergey V Shmelkov*, Lin Jun, Ryan St. Clair, Deirdre McGarrigle, Christopher A Derderian, Jaroslav K Usenko, Carla Costa, Fan Zhang, Xinzheng Guo, and Shahin Rafii Department of Medicine, Division of Hematology-Oncology, Weill Medical College of Cornell University, New York, NY, USA * Corresponding author; email: svs2001{at}med.cornell.edu. AC133 is a member of a novel family of cell surface proteins with five transmembrane domains. The function of AC133 is unknown. Although AC133 mRNA is detected in different tissues, its expression in the hematopoietic system is restricted to CD34+ stem cells. AC133 is also expressed on stem cells of other tissues, including endothelial progenitor cells. However, despite the potential importance of AC133 to the field of stem cell biology, nothing is known about the transcriptional regulation of AC133 expression. In this report we showed that the human AC133 gene has at least nine distinctive 5'-UTR exons, resulting in the formation of at least seven alternatively spliced 5'-UTR isoforms of AC133 mRNA, which are expressed in a tissue dependent manner. We found that transcription of these AC133 isoforms is controlled by five alternative promoters, and we demonstrated their activity on AC133 expressing cell lines using a luciferase reporter system. We also showed that in vitro methylation of two of these AC133 promoters completely suppresses their activity, suggesting that methylation plays a role in their regulation. Identification of tissue specific AC133 promoters may provide a novel method to isolate tissue-specific stem and progenitor cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Yanagisawa, I. Kadouchi, K. Yokomori, M. Hirose, M. Hakozaki, H. Hojo, K. Maeda, E. Kobayashi, and T. Murakami Identification and Metastatic Potential of Tumor-Initiating Cells in Malignant Rhabdoid Tumor of the Kidney Clin. Cancer Res., May 1, 2009; 15(9): 3014 - 3022. [Abstract] [Full Text] [PDF] T. Jiang, B. J. Collins, N. Jin, D. N. Watkins, M. V. Brock, W. Matsui, B. D. Nelkin, and D. W. Ball Achaete-Scute Complex Homologue 1 Regulates Tumor-Initiating Capacity in Human Small Cell Lung Cancer Cancer Res., February 1, 2009; 69(3): 845 - 854. [Abstract] [Full Text] [PDF] D. J. Vander Griend, W. L. Karthaus, S. Dalrymple, A. Meeker, A. M. DeMarzo, and J. T. Isaacs The Role of CD133 in Normal Human Prostate Stem Cells and Malignant Cancer-Initiating Cells Cancer Res., December 1, 2008; 68(23): 9703 - 9711. [Abstract] [Full Text] [PDF] J. M. Yi, H.-C. Tsai, S. C. Glockner, S. Lin, J. E. Ohm, H. Easwaran, C. D. James, J. F. Costello, G. Riggins, C. G. Eberhart, et al. Abnormal DNA Methylation of CD133 in Colorectal and Glioblastoma Tumors Cancer Res., October 1, 2008; 68(19): 8094 - 8103. [Abstract] [Full Text] [PDF] J. Torrisani, N. Hanoun, H. Laurell, F. Lopez, J.-J. Maoret, A. Souque, C. Susini, P. Cordelier, and L. Buscail Identification of an Upstream Promoter of the Human Somatostatin Receptor, hSSTR2, Which Is Controlled by Epigenetic Modifications Endocrinology, June 1, 2008; 149(6): 3137 - 3147. [Abstract] [Full Text] [PDF] V. Coskun, H. Wu, B. Blanchi, S. Tsao, K. Kim, J. Zhao, J. C. Biancotti, L. Hutnick, R. C. Krueger Jr., G. Fan, et al. From the Cover: CD133+ neural stem cells in the ependyma of mammalian postnatal forebrain PNAS, January 22, 2008; 105(3): 1026 - 1031. [Abstract] [Full Text] [PDF] C. Igreja, M. Courinha, A. S. Cachaco, T. Pereira, J. Cabecadas, M. G. da Silva, and S. Dias Characterization and clinical relevance of circulating and biopsy-derived endothelial progenitor cells in lymphoma patients Haematologica, April 1, 2007; 92(4): 469 - 477. [Abstract] [Full Text] [PDF] S. Bruno, B. Bussolati, C. Grange, F. Collino, M. E. Graziano, U. Ferrando, and G. Camussi CD133+ Renal Progenitor Cells Contribute to Tumor Angiogenesis Am. J. Pathol., December 1, 2006; 169(6): 2223 - 2235. [Abstract] [Full Text] [PDF] Y. Zong, S. Zhou, S. Fatima, and B. P. Sorrentino Expression of Mouse Abcg2 mRNA during Hematopoiesis Is Regulated by Alternative Use of Multiple Leader Exons and Promoters J. Biol. Chem., October 6, 2006; 281(40): 29625 - 29632. [Abstract] [Full Text] [PDF] J.-F. Gagnon, O. Bernard, L. Villeneuve, B. Tetu, and C. Guillemette Irinotecan Inactivation Is Modulated by Epigenetic Silencing of UGT1A1 in Colon Cancer Clin. Cancer Res., March 15, 2006; 12(6): 1850 - 1858. [Abstract] [Full Text] [PDF] M. Thomas, A. Gessner, H.-P. Vornlocher, P. Hadwiger, J. Greil, and O. Heidenreich Targeting MLL-AF4 with short interfering RNAs inhibits clonogenicity and engraftment of t(4;11)-positive human leukemic cells Blood, November 15, 2005; 106(10): 3559 - 3566. [Abstract] [Full Text] [PDF] S. V. Shmelkov, S. Meeus, N. Moussazadeh, P. Kermani, W. K. Rashbaum, S. Y. Rabbany, M. A. Hanson, W. J. Lane, R. St. Clair, K. A. Walsh, et al. Cytokine Preconditioning Promotes Codifferentiation of Human Fetal Liver CD133+ Stem Cells Into Angiomyogenic Tissue Circulation, March 8, 2005; 111(9): 1175 - 1183. [Abstract] [Full Text] [PDF] L. De Felice, C. Tatarelli, M. G. Mascolo, C. Gregorj, F. Agostini, R. Fiorini, V. Gelmetti, S. Pascale, F. Padula, M. T. Petrucci, et al. Histone Deacetylase Inhibitor Valproic Acid Enhances the Cytokine-Induced Expansion of Human Hematopoietic Stem Cells Cancer Res., February 15, 2005; 65(4): 1505 - 1513. [Abstract] [Full Text] [PDF] B. Bussolati, S. Bruno, C. Grange, S. Buttiglieri, M. C. Deregibus, D. Cantino, and G. Camussi Isolation of Renal Progenitor Cells from Adult Human Kidney Am. J. Pathol., February 1, 2005; 166(2): 545 - 555. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020