SEARCH:   Advanced

Blood, 1 March 2004, Vol. 103, No. 5, pp. 1685-1692. Prepublished online as a Blood First Edition Paper on October 30, 2003; DOI 10.1182/blood-2003-06-1921. This Article Full Text (PDF) All Versions of this Article: 2003-06-1921v1 103/5/1685    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Winkler, I. G Articles by Levesque, J.-P. Search for Related Content PubMed PubMed Citation Articles by Winkler, I. G Articles by Levesque, J.-P. Social Bookmarking                   What's this? Submitted June 16, 2003 Accepted October 20, 2003 Adhesion to E-selectin promotes growth inhibition and apoptosis of human and murine hematopoietic progenitor cells independent of PSGL-1 Ingrid G Winkler*, Karen R Snapp, Paul J Simmons, and Jean-Pierre Levesque Adhesive Interactions and Cell Trafficking Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia; Stem Cell Biology Program, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia Department of Pharmacology, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA * Corresponding author; email: ingrid.winkler{at}petermac.org. Although both P- and E-selectin are constitutively expressed on bone marrow endothelial cells, their role in the regulation of hematopoiesis has only recently been investigated. We have previously shown that P-selectin glycoprotein ligand-l (PSGL-1/CD162) is expressed by primitive human bone marrow CD34+ cells, mediates their adhesion to P-selectin and more importantly, inhibits their proliferation. We now demonstrate that adhesion to E-selectin inhibits the proliferation of human CD34+ cells isolated either from human umbilical cord blood, adult mobilized blood or steady-state bone marrow. Furthermore a subpopulation, that does not contain the most primitive hematopoietic progenitor cells, undergoes apoptosis following E-selectin-mediated adhesion. The same phenomenon was observed in cells isolated from mouse bone marrow. Using lineage-negative Sca-1+ c-KIT+ bone marrow cells from PSGL-1-/- and wildtype mice, we establish that PSGL-1 is not the ligand involved in E-selectin-mediated growth inhibition and apoptosis. Moreover, stable transfection of the human myeloid cell line K562 (which does not express PSGL-1), with (1,3) fucosyltransferase VII alone was sufficient to recapitulate the E-selectin-mediated growth inhibition and apoptosis observed in hematopoietic progenitor cells. These data demonstrate that E-selectin ligand(s) other than PSGL-1 transduce growth inhibitory and pro-apoptotic signals and require post-translational fucosylation to be functional. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Martinez, M. Joffraud, S. Giraud, B. Baisse, M. P. Bernimoulin, M. Schapira, and O. Spertini Regulation of PSGL-1 Interactions with L-selectin, P-selectin, and E-selectin: ROLE OF HUMAN FUCOSYLTRANSFERASE-IV AND -VII J. Biol. Chem., February 18, 2005; 280(7): 5378 - 5390. [Abstract] [Full Text] [PDF] A. Hidalgo and P. S. Frenette Enforced fucosylation of neonatal CD34+ cells generates selectin ligands that enhance the initial interactions with microvessels but not homing to bone marrow Blood, January 15, 2005; 105(2): 567 - 575. [Abstract] [Full Text] [PDF] L. Xia, J. M. McDaniel, T. Yago, A. Doeden, and R. P. McEver Surface fucosylation of human cord blood cells augments binding to P-selectin and E-selectin and enhances engraftment in bone marrow Blood, November 15, 2004; 104(10): 3091 - 3096. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020