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Blood, 1 March 2004, Vol. 103, No. 5, pp. 1647-1652. Prepublished online as a Blood First Edition Paper on November 26, 2003November 20, 2003; DOI 10.1182/blood-2003-06-1967. This Article Full Text (PDF) All Versions of this Article: 2003-06-1967v1 2003-06-1967v2 103/5/1647    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pochampally, R. R Articles by Prockop, D. J Search for Related Content PubMed PubMed Citation Articles by Pochampally, R. R Articles by Prockop, D. J Social Bookmarking                   What's this? Submitted June 19, 2003 Accepted October 11, 2003 Serum deprivation of human marrow stromal cells (hMSCs) selects for a sub-population of early progenitor cells with enhanced expression of Oct-4 and other embryonic genes Radhika R Pochampally, Jason R Smith, Joni Ylostalo, and Darwin J Prockop* Center for Gene Therapy, Tulane University Medical Center, New Orleans, LA, USA * Corresponding author; email: dprocko{at}tulane.edu. Recently there has been interest in developing cell and gene therapies with adult stem cells from human bone marrow referred to as mesenchymal stem cells or marrow stromal cells (hMSCs). We incubated early passage hMSCs in serum-free medium without cytokines or other supplements for 2 to 4 wks. Surprisingly, a sub-population of the cells survived serum deprivation and then began to proliferate in serum-containing medium. The cells selected by serum-deprivation had longer telomeres than control cells. Also, the patterns of gene expression revealed by RT-PCR assays and microarray data indicated that the cells selected by serum deprivation were a sub-population of very early progenitor cells with enhanced expression of Oct-4 and several other genes characteristically expressed in embryonic cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Z. Ratajczak, D.-M. Shin, and M. Kucia Very Small Embryonic/Epiblast-Like Stem Cells: A Missing Link To Support the Germ Line Hypothesis of Cancer Development? Am. J. Pathol., June 1, 2009; 174(6): 1985 - 1992. [Abstract] [Full Text] [PDF] F. Ng, S. Boucher, S. Koh, K. S. R. Sastry, L. Chase, U. Lakshmipathy, C. Choong, Z. Yang, M. C. Vemuri, M. S. Rao, et al. 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