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Blood, 1 April 2004, Vol. 103, No. 7, pp. 2677-2682. Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-06-2070. This Article Full Text (PDF) All Versions of this Article: 2003-06-2070v1 103/7/2677    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jedema, I. Articles by Falkenburg, J H F. Search for Related Content PubMed PubMed Citation Articles by Jedema, I. Articles by Falkenburg, J H F. Social Bookmarking                   What's this? Submitted June 24, 2003 Accepted November 13, 2003 A new CFSE-based assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell population Inge Jedema*, Nicole M van der Werff, Renee M Y Barge, Roel Willemze, and J H Frederik Falkenburg Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands * Corresponding author; email: i.jedema{at}lumc.nl. For the clinical evaluation of the efficacy of cellular immunotherapy it is necessary to analyze the effector functions of T cells against primary leukemic target cell populations which are usually considerably heterogeneous caused by differential maturations stages of the leukemic cells. An appropriate assay should not only allow the quantitative analysis of rapid cell death induction as measured by the conventional 51Cr release assay, but also of the more slowly executing pathways of T cell induced apoptosis occurring within days instead of hours which cannot be measured using this method. Furthermore, it should dissect the differential susceptibility to T cell induced cell death of various target cell subpopulations, and characterize the malignant precursor cells capable of producing malignant progeny. To fulfill these requirements we developed a new assay based on CFSE labeling of the target cell population combined with antibody staining of specific cell populations and addition of fluorescent microbeads to quantitatively monitor target cell death occurring within a longer time-frame up to at least 5 days, facilitating the analysis of differential recognition of distinct cell types within a heterogeneous target cell population, and allowing simultaneously evaluation of the proliferative status of surviving target cells in response to relevant cytokines. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Figueiredo, M. Wittmann, D. Wang, R. Dressel, A. Seltsam, R. Blasczyk, and B. Eiz-Vesper Heat shock protein 70 (HSP70) induces cytotoxicity of T-helper cells Blood, March 26, 2009; 113(13): 3008 - 3016. [Abstract] [Full Text] [PDF] W. J. Norde, I. M. Overes, F. Maas, H. Fredrix, J. C. M. Vos, M. G. 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