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Blood, 15 April 2004, Vol. 103, No. 8, pp. 3073-3075. Prepublished online as a Blood First Edition Paper on January 8, 2004; DOI 10.1182/blood-2003-07-2305. This Article Full Text (PDF) All Versions of this Article: 2003-07-2305v1 103/8/3073    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dyer, C. M Articles by Lew, A. M Search for Related Content PubMed PubMed Citation Articles by Dyer, C. M Articles by Lew, A. M Social Bookmarking                   What's this? Submitted July 10, 2003 Accepted November 18, 2003 Unexpectedly, induction of cytotoxic T lymphocytes enhanced the humoral response after DNA immunization Christopher M Dyer, Yifan Zhan, Jamie L Brady, Francis R Carbone, Mark J Smyth, and Andrew M Lew* Autoimmunity & Transplantation, Walter and Eliza Hall Institute of Medical Research, Melbourne, Vic, Australia Microbiology & Immunology, University of Melbourne, Melbourne, Vic, Australia Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, Vic, Australia * Corresponding author; email: lew{at}wehi.edu.au. Although there are many examples (e.g. immune deviation) where enhanced cellular responses correspond with lower humoral responses, here we demonstrate for the first time two models in which cytotoxic T lymphocyte (CTL) activity is associated with an enhanced antibody response. Firstly, C57BL/6 mice generate a stronger antibody response to ovalbumin DNA immunization than congenic bm1 mice. The latter differ from C57BL/6 mice in that the H-2Kb molecule is mutated so that the immunodominant CTL epitope of ovalbumin is no longer presented. Secondly, pre-existing CTLs (induced by ovalbumin peptide-priming) increased the antibody response to a second unrelated antigen (-galactosidase) co-immunized with ovalbumin. One possible mechanism is that CTLs may release antigen from DNA transfected cells by killing or damaging them, and this freed antigen is then accessible to dendritic cells and B cells. Our finding of CTL-mediated antibody enhancement has important implications for tumor and viral immunobiology and vaccination. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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