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Blood, 1 January 2005, Vol. 105, No. 1, pp. 215-218. Prepublished online as a Blood First Edition Paper on June 10, 2004; DOI 10.1182/blood-2003-07-2367. This Article Full Text (PDF) All Versions of this Article: 2003-07-2367v1 105/1/215    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Solanilla, A. Articles by Ripoche, J. Search for Related Content PubMed PubMed Citation Articles by Solanilla, A. Articles by Ripoche, J. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 15, 2003 Accepted April 28, 2004 Platelet-associated CD154 in immune thrombocytopenic purpura Anne Solanilla, Jean-Max Pasquet, Jean-Francois Viallard, Cecile Contin, Christophe Grosset, Julie Dechanet-Merville, Maryse Dupouy, Marc Landry, Francis Belloc, Paquita Nurden, Patrick Blanco, Jean-Francois Moreau, Jean-Luc Pellegrin, Alan Nurden, and Jean Ripoche* CNRS FRE 2617, Universite de Bordeaux2, Bordeaux, France Service de Medecine Interne, Hopital du Haut-Leveque, Pessac, France UMR 5540, Universite de Bordeaux2, Bordeaux, France Laboratoire d'Hematologie, Hopital Cardiologique, Pessac, France * Corresponding author; email: Jean.Ripoche{at}umr5540.u-bordeaux2.fr. CD40-ligand (CD154) is expressed on activated CD4+ T-lymphocytes and is essential for the T cell-dependent activation of B-lymphocytes. CD154 is also expressed at the activated platelet surface. In this study, we show that platelet-associated CD154 is increased in immune thrombocytopenic purpura (ITP), a disease characterized by an auto-immune response against proteins of the platelet membrane. CD154 and its messenger RNA were also present in increased amounts in the megakaryocytes of patients with ITP. We found that platelet-associated CD154 is competent to induce the CD40-dependent proliferation of B-lymphocytes and we observed an in vitro CD154-dependent production of antibodies to the GPIIb/IIIa complex (integrin IIb3) when platelets and peripheral blood B lymphocytes from ITP patients with circulating anti-GPIIb/IIIa antibody were cultured together. Therefore, platelet-associated CD154 expression is increased in ITP and is able to drive the activation of auto-reactive B-lymphocytes in this disease. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. L. Sprague, B. D. Elzey, S. A. Crist, T. J. Waldschmidt, R. J. Jensen, and T. L. Ratliff Platelet-mediated modulation of adaptive immunity: unique delivery of CD154 signal by platelet-derived membrane vesicles Blood, May 15, 2008; 111(10): 5028 - 5036. [Abstract] [Full Text] [PDF] N. Li Platelet-lymphocyte cross-talk J. Leukoc. Biol., May 1, 2008; 83(5): 1069 - 1078. [Abstract] [Full Text] [PDF] B. D. Elzey, N. W. Schmidt, S. A. Crist, T. P. Kresowik, J. T. Harty, B. Nieswandt, and T. L. Ratliff Platelet-derived CD154 enables T-cell priming and protection against Listeria monocytogenes challenge Blood, April 1, 2008; 111(7): 3684 - 3691. [Abstract] [Full Text] [PDF] K Schallmoser, C Rosin, R Vormittag, M Brunner, D Dunkler, I Pabinger, and S Panzer Specificities of Platelet Autoantibodies and Platelet Activation in Lupus Anticoagulant Patients: A Relation to their History of Thromboembolic Disease Lupus, August 1, 2006; 15(8): 507 - 514. [Abstract] [PDF] D. S. Beardsley ITP in the 21st Century Hematology, January 1, 2006; 2006(1): 402 - 407. [Abstract] [Full Text] [PDF] B. D. Elzey, J. F. Grant, H. W. Sinn, B. Nieswandt, T. J. Waldschmidt, and T. L. Ratliff Cooperation between platelet-derived CD154 and CD4+ T cells for enhanced germinal center formation J. Leukoc. Biol., July 1, 2005; 78(1): 80 - 84. [Abstract] [Full Text] [PDF]

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