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Blood, 15 March 2004, Vol. 103, No. 6, pp. 2062-2070. Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-07-2388. This Article Full Text (PDF) All Versions of this Article: 2003-07-2388v1 103/6/2062    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bond, H. M Articles by Venuta, S. Search for Related Content PubMed PubMed Citation Articles by Bond, H. M Articles by Venuta, S. Social Bookmarking                   What's this? Submitted July 16, 2003 Accepted October 29, 2003 Early hematopoietic zinc finger protein (EHZF), the human homologue to mouse Evi3, is highly expressed in primitive human hematopoietic cells Heather M Bond, Maria Mesuraca, Ennio Carbone, Patrizia Bonelli, Valter Agosti, Nicola Amodio, Gennaro De Rosa, Massimo Di Nicola, Alessandro M Gianni, Malcolm A S Moore, Akiko Hata, Michele Grieco, Giovanni Morrone*, and Salvatore Venuta Department of Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, NY, USA; Department of Experimental and Clinical Medicine, University of Catanzaro, Catanzaro, Italy Department of Experimental Oncology, National Cancer Institute Fondazione G. Pascale, Naples, Italy Clinical Hematology Divison, University of Napoli Federico II, Naples, Italy Cristina Gandini Transplantation Unit, Istituto Nazionale Tumori, Milan, Italy Molecular Cardiology Research Institute, New England Medical Center, Tufts University School of Medicine, Boston, MA, USA * Corresponding author; email: morrone{at}unicz.it. Comparison of the gene expression repertoire in human hematopoietic progenitors and mature leukocytes led to identification of a transcript expressed in CD34+ cells and undetectable in differentiated cells. Sequencing of the cDNA (termed EHZF: early hematopoietic zinc finger) revealed 30 zinc fingers with 96% homology to mouse Evi3, a recently identified gene associated with the retroviral integration site in AKXD-27 B-cell lymphomas. EHZF and Evi3 share high homology with the co-transcription factor Oaz, implicated in the control of olfactory epithelium and B-lymphocyte differentiation, and in the bone morphogenic proteins (BMPs) signal transduction. Here we show that: a) EHZF expression is abundant in human CD34+ progenitors and declines rapidly during cytokine-driven differentiation; b) significant mRNA levels are found in the majority of acute myelogenous leukemias; c) in response to BMPs EHZF complexes SMADs 1 and 4, binds to, and enhances the transcriptional activity of, a BMP2/4 responsive element; d) EHZF inhibits the transcriptional activity of Early B-cell Factor (EBF), a transcription factor essential for specification of the B cell lineage. Taken together, our data suggest that EHZF is likely to play a relevant role in the control of human hematopoiesis and might be implicated in the development of hematopoietic malignancies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. La Rocca, M. Fulciniti, T. Lakshmikanth, M. Mesuraca, T. H. Ali, V. Mazzei, N. Amodio, L. Catalano, B. Rotoli, O. Ouerfelli, et al. Early Hematopoietic Zinc Finger Protein Prevents Tumor Cell Recognition by Natural Killer Cells J. Immunol., April 15, 2009; 182(8): 4529 - 4537. [Abstract] [Full Text] [PDF] E. Matsubara, I. Sakai, J. Yamanouchi, H. Fujiwara, Y. Yakushijin, T. Hato, K. Shigemoto, and M. Yasukawa The Role of Zinc Finger Protein 521/Early Hematopoietic Zinc Finger Protein in Erythroid Cell Differentiation J. Biol. Chem., February 6, 2009; 284(6): 3480 - 3487. [Abstract] [Full Text] [PDF] K. L. Bennett, M. Karpenko, M.-t. Lin, R. Claus, K. Arab, G. Dyckhoff, P. Plinkert, E. Herpel, D. Smiraglia, and C. Plass Frequently Methylated Tumor Suppressor Genes in Head and Neck Squamous Cell Carcinoma Cancer Res., June 15, 2008; 68(12): 4494 - 4499. [Abstract] [Full Text] [PDF] G. K. McEwen, A. Woolfe, D. Goode, T. Vavouri, H. Callaway, and G. Elgar Ancient duplicated conserved noncoding elements in vertebrates: A genomic and functional analysis Genome Res., April 1, 2006; 16(4): 451 - 465. [Abstract] [Full Text] [PDF] M. Ku, S. Howard, W. Ni, G. Lagna, and A. Hata OAZ Regulates Bone Morphogenetic Protein Signaling through Smad6 Activation J. Biol. Chem., February 24, 2006; 281(8): 5277 - 5287. [Abstract] [Full Text] [PDF]

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