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Blood, 1 May 2004, Vol. 103, No. 9, pp. 3287-3295. Prepublished online as a Blood First Edition Paper on January 15, 2004; DOI 10.1182/blood-2003-07-2428. This Article Full Text (PDF) All Versions of this Article: 2003-07-2428v1 103/9/3287    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Carlo-Stella, C. Articles by Gianni, A. M Search for Related Content PubMed PubMed Citation Articles by Carlo-Stella, C. Articles by Gianni, A. M Social Bookmarking                   What's this? Submitted July 18, 2003 Accepted January 5, 2004 Use of Recombinant Human Growth Hormone (rhGH) plus Recombinant Human Granulocyte Colony-Stimulating Factor (rhG-CSF) for the Mobilization and Collection of CD34+ Cells in Poor Mobilizers Carmelo Carlo-Stella*, Massimo Di Nicola, Raffaella Milani, Anna Guidetti, Michele Magni, Marco Milanesi, Paolo Longoni, Paola Matteucci, Franca Formelli, Fernando Ravagnani, Paolo Corradini, and Alessandro M Gianni Cristina Gandini Medical Oncology Unit, Istituto Nazionale Tumori, Milano, Italy; Chair of Medical Oncology, University of Milano, Milano, Italy Cristina Gandini Medical Oncology Unit, Istituto Nazionale Tumori, Milano, Italy Hematology and Bone Marrow Transplantation Unit, Istituto Nazionale Tumori, Milano, Italy Department of Experimental Oncology, Istituto Nazionale Tumori, Milano, Italy Division of Immunohematology and Transfusion Medicine, Istituto Nazionale Tumori, Milano, Italy Hematology and Bone Marrow Transplantation Unit, Istituto Nazionale Tumori, Milano, Italy; Chair of Hematology, University of Milano, Milano, Italy * Corresponding author; email: carmelo.carlostella{at}unimi.it. The activity of recombinant human growth hormone (rhGH) in enhancing CD34+ cell mobilization elicited by chemotherapy plus granulocyte colony-stimulating factor (rhG-CSF) was evaluated in 16 hard-to-mobilize patients, i.e., those achieving a peak of circulating CD34+ cells 10/µL, or a collection of CD34+ cells 2 x 106/kg. Patients who had failed a first mobilization attempt with chemotherapy plus rhG-CSF (5 µg/kg/day) were re-mobilized with chemotherapy plus rhG-CSF and rhGH (100 µg/kg/day). As compared with rhG-CSF, the combined rhGH/rhG-CSF treatment induced significantly higher (P .05) median peak values for CD34+ cells/[mu]L (7 vs 29), CFCs/ml (2,154 vs 28,510), and LTC-IC/ml (25 vs 511). Following rhG-CSF and rhGH/rhG-CSF, the median yields of CD34+ cells per leukapheresis were 1.1 x 106/kg and 2.3 x 106/kg (P .008), respectively; the median total collection of CD34+ cells were 1.1 x 106/kg and 6 x 106/kg (P .008), respectively. No specific side effect could be ascribed to rhGH, except a transient hyperglycemia occurring in two patients. Reinfusion of rhGH/rhG-CSF-mobilized cells following myeloablative therapy resulted in prompt hematopoietic recovery. In conclusion, our data demonstrate that in poor mobilizers addition of rhGH to rhG-CSF allows to efficiently mobilize and collect CD34+ cells with maintained functional properties. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. A van der Klaauw, A. M Pereira, T. J Rabelink, E. P M Corssmit, A.-J. Zonneveld, H. Pijl, H. C de Boer, J. W A Smit, J. A Romijn, and E. J P de Koning Recombinant human GH replacement increases CD34+ cells and improves endothelial function in adults with GH deficiency Eur. J. Endocrinol., August 1, 2008; 159(2): 105 - 111. [Abstract] [Full Text] [PDF] D. Focosi, R. E. Kast, S. Galimberti, and M. Petrini Conditioning response to granulocyte colony-stimulating factor via the dipeptidyl peptidase IV-adenosine deaminase complex J. Leukoc. Biol., August 1, 2008; 84(2): 331 - 337. [Abstract] [Full Text] [PDF] T. Papayannopoulou and D. T. Scadden Stem-cell ecology and stem cells in motion Blood, April 15, 2008; 111(8): 3923 - 3930. [Abstract] [Full Text] [PDF] R. M. Lemoli and A. D'Addio Hematopoietic stem cell mobilization Haematologica, March 1, 2008; 93(3): 321 - 324. [Full Text] [PDF] O. M. Pello, M. del Carmen Moreno-Ortiz, J. M. Rodriguez-Frade, L. Martinez-Munoz, D. Lucas, L. Gomez, P. Lucas, E. Samper, M. Aracil, C. Martinez-A, et al. SOCS up-regulation mobilizes autologous stem cells through CXCR4 blockade Blood, December 1, 2006; 108(12): 3928 - 3937. [Abstract] [Full Text] [PDF]

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