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Blood, 1 April 2004, Vol. 103, No. 7, pp. 2522-2529. Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-07-2439. This Article Full Text (PDF) All Versions of this Article: 2003-07-2439v1 103/7/2522    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lorsbach, R. B Articles by Downing, J. R Search for Related Content PubMed PubMed Citation Articles by Lorsbach, R. B Articles by Downing, J. R Social Bookmarking                   What's this? Submitted July 18, 2003 Accepted November 19, 2003 Role of Runx1 in adult hematopoiesis: analysis of Runx1-IRES-GFP knock-in mice reveals differential lineage expression Robert B Lorsbach*, Jennifer Moore, Sonny O Ang, Weili Sun, Noel Lenny, and James R Downing Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN, USA * Corresponding author; email: robert.lorsbach{at}stjude.org. The Runx1/Core Binding Factor- (CBF) transcriptional complex is required for the establishment of hematopoiesis during development. Despite its critical role during development, a detailed analysis of Runx1 expression within specific lineages and developmental stages of the adult hematopoietic system is lacking. To address this, we have developed a Runx1-green fluorescent protein (GFP) knock-in mouse. We show that Runx1 is expressed in several hematopoietic lineages, including myeloid, B lymphoid and T lymphoid cells. By contrast, Runx1 is weakly expressed in early erythroid cells, and its expression is rapidly extinguished during later stages of erythropoiesis. Runx1 expression is induced during early B cell development and is expressed at a uniform level during all subsequent stages of B cell development. Within the thymus, Runx1-GFP is expressed at the highest level in CD4-CD8- double negative thymocytes. In peripheral T cells, Runx1 is differentially expressed, with CD4+ T cells expressing 2-3 fold higher levels of Runx1 than CD8+ cells. Taken together, these findings indicate that although widely expressed in the hematopoietic system, the expression of Runx1 is regulated in a cell-type and maturation-stage specific manner. In addition, the Runx1-IRES-GFP knock-in mouse strain should prove valuable for investigation of Runx1 function in adult hematopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. 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