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Blood, 1 February 2004, Vol. 103, No. 3, pp. 1011-1019. Prepublished online as a Blood First Edition Paper on October 2, 2003; DOI 10.1182/blood-2003-07-2449. This Article Full Text (PDF) All Versions of this Article: 2003-07-2449v1 103/3/1011    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Leen, A. M Articles by Rooney, C. M Search for Related Content PubMed PubMed Citation Articles by Leen, A. M Articles by Rooney, C. M Social Bookmarking                   What's this? Submitted July 21, 2003 Accepted September 28, 2003 Fiber-modified adenoviruses generate sub-group cross-reactive, adenovirus-specific cytotoxic T lymphocytes for therapeutic applications Ann M Leen, Uluhan Sili, Barbara Savoldo, Alan M Jewell, Pedro A Piedra, Malcolm K Brenner, and Cliona M Rooney* Department of Pediatrics and Medicine, Center for Cell and Gene Therapy, Houston, Texas, USA Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA Department of Pediatrics and Medicine, Center for Cell and Gene Therapy, Houston, Texas, USA; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA * Corresponding author; email: cmrooney{at}texaschildrenshospital.org. Adenovirus (Ad) infections are responsible for considerable morbidity and mortality, particularly in pediatric hematopoietic stem cell transplant (HSCT) recipients. To date there is no therapy. The present study was motivated by the potential for using adoptive immunotherapy as either prophylaxis or treatment for Ad infections and associated diseases. The authors have developed a protocol to reactivate Ad-specific memory T cells from peripheral blood mononuclear cells (PBMCs), using a clinical grade adenoviral vector. Such lines contain a specific CD4 and CD8 T cell component, and are capable of recognizing and lysing target cells infected with wild-type Ad serotypes from different Ad groups. Furthermore, the frequency of Ad-specific precursors can be determined in PBMCs ex vivo, and used as a means to assess changes in Ad-specific T cell memory responses post-infusion. This is the first report of a simple and reproducible method to activate and expand Ad-specific cytotoxic T lymphocytes (CTL), which should be protective against the range of different Ad subtypes that affect transplant recipients. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. M. Leen, A. Christin, G. D. Myers, H. Liu, C. R. Cruz, P. J. Hanley, A. A. Kennedy-Nasser, K. S. Leung, A. P. Gee, R. A. Krance, et al. Cytotoxic T lymphocyte therapy with donor T cells prevents and treats adenovirus and Epstein-Barr virus infections after haploidentical and matched unrelated stem cell transplantation Blood, November 5, 2009; 114(19): 4283 - 4292. [Abstract] [Full Text] [PDF] M. Echavarria Adenoviruses in Immunocompromised Hosts Clin. Microbiol. Rev., October 1, 2008; 21(4): 704 - 715. [Abstract] [Full Text] [PDF] A. M. Leen, A. Christin, M. Khalil, H. Weiss, A. P. Gee, M. K. Brenner, H. E. Heslop, C. M. Rooney, and C. M. Bollard Identification of Hexon-Specific CD4 and CD8 T-Cell Epitopes for Vaccine and Immunotherapy J. Virol., January 1, 2008; 82(1): 546 - 554. [Abstract] [Full Text] [PDF] B. Savoldo, C. M. Rooney, A. Di Stasi, H. 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